Assessment of Trabecular Bone Architecture and Intrinsic Properties of Cortical bone Tissue in a Mouse Model of Chronic Kidney Disease
Assessment of Trabecular Bone Architecture and Intrinsic Properties of Cortical bone Tissue in a Mouse Model of Chronic Kidney Disease
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DOI：
10.2485/jhtb.20.79
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发表时间：
2011
影响因子：
0.4
通讯作者：
Syotaro Kadokawa;Takeshi Matsumoto;H. Naito;Masao Tanaka
中科院分区：
文献类型：
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作者：
Syotaro Kadokawa;Takeshi Matsumoto;H. Naito;Masao Tanaka
慢性肾脏疾病（CKD）通常与骨折风险增加有关；然而，ckd对骨微观结构和内在材料特性的影响尚未完全阐明。本研究旨在评估高周转率CKD小鼠模型胫骨骨小梁结构变化的时间过程和皮质骨的材料特性。采用5/6次肾切除的雄性小鼠（Nx, n=8）和假手术的雄性小鼠（Sham, n=8）。从8周龄（术后2周）开始，给Nx小鼠喂食高磷饲料。10 ~ 26周龄，每隔4周对胫骨干骺端进行体内微计算机断层扫描（μCT）进行骨小梁结构分析。然后，在献祭前对动物进行血液采样，并采集扫描后的胫骨。通过傅里叶变换红外显微光谱法评估皮质横截面中间层的矿物与基质比率、矿物成熟度和胶原成熟度，并通过纳米压痕测试评估硬度和硬度。血清化学物质显示Nx成功诱导高周转率CKD。两组小梁网络均出现年龄相关的退化，但Nx的退化程度更高。体内μCT显示年龄与CKD在小梁数量、厚度和分离上有显著的相互作用。另一方面，皮质骨的化学和力学性能在Nx和Sham之间没有显著差异。因此，小梁结构受损将导致高周转率CKD的骨脆性。
Chronic kidney disease (CKD) is often associated with increased fracture risk; however, the CKD-effects on bone microstructure and intrinsic material properties have not been fully elucidated. This study was undertaken to evaluate the time course of trabecular architectural changes and material properties of cortical bone in the tibia of a mouse model of high-turnover CKD. Male mice subjected to 5/6 nephrectomy (Nx, n=8) and those sham-operated (Sham, n=8) were used. Starting at 8 weeks of age (2 weeks after surgery), Nx mice were fed with high phosphate diet. The tibial metaphysis was scanned by in vivo micro-computed tomography (μCT) for trabecular architecture analysis from 10 to 26 weeks of age with 4-week interval. Then, the animal was blood-sampled before sacrifice, and the scanned tibia was harvested. The midlayer of a cortical cross-section was evaluated for mineral-to-matrix ratio, mineral maturity, and collagen maturity by Fourier transform infrared microspectroscopy and for stiffness and hardness by the nanoindentation test. Serum chemicals showed the successful induction of high-turnover CKD in Nx. The age-related regression of trabecular network was observed in both groups but to the higher degree in Nx. In vivo μCT revealed significant interactions between age and CKD in trabecular number, thickness, and separation. On the other hand, the chemical and mechanical properties of cortical bone did not differ significantly between Nx and Sham. Thus, the compromised trabecular architecture will be contributory to bone fragility in high-turnover CKD.