Genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients.
Genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients.
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DOI：
10.3402/jom.v3i0.6362
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发表时间：
2011
影响因子：
4.5
通讯作者：
Haase EM
中科院分区：
文献类型：
--
作者：
Heo SM;Sung RS;Scannapieco FA;Haase EM
白色念珠菌作为一种无害的口腔微生物常存在于健康人的口腔中。这种机会性真菌可在重症患者中引起重大疾病，例如在重症监护室（ICU）中接受机械通气的患者，其局部气道防御机制受损。本研究的目的是确定患者内和患者间的遗传关系之间的菌株C。经支气管肺泡灌洗，在接受机械通气的病人的牙菌斑、气管分泌物和下呼吸道中回收了白色念珠菌。采用脉冲场凝胶电泳（PFGE）分型方法，分析了3种不同类型人群的亲缘关系。白念珠菌菌株，包括电泳核型分析（EK）和使用SfiI（REAG-S）和BssHII（REAG-B）的基因组限制性内切酶分析。梭来自同一患者的牙菌斑和气管-支气管部位的白色念珠菌分离物在遗传上是不可区分的，并且随着时间的推移而保留，而来自不同患者的菌株通常分成不同的基因型。在这三种方法中，REAG-B被证明是区分菌株的最具区分性的方法。从同一患者的口腔和气管支气管部位发现遗传相似的菌株支持了口腔可能是C.白色念珠菌扩散至机械通气患者的气管和肺。
Candida albicans often resides in the oral cavity of healthy humans as a harmless commensal organism. This opportunistic fungus can cause significant disease in critically ill patients, such as those undergoing mechanical ventilation in the intensive care unit (ICU) having compromised local airway defense mechanisms. The goal of this study was to determine the intra- and inter-patient genetic relationship between strains of C. albicans recovered from dental plaque, tracheal secretions, and the lower airway by bronchoalveolar lavage of patients undergoing mechanical ventilation. Three pulsed-field gel electrophoresis (PFGE) typing methods were used to determine the genetic relatedness of the C. albicans strains, including electrophoretic karyotyping (EK) and restriction endonuclease analysis of the genome using SfiI (REAG-S) and BssHII (REAG-B). The C. albicans isolates from dental plaque and tracheo-bronchial sites from the same patient were genetically indistinguishable and retained over time, whereas strains from different patients usually separated into different genotypes. Among the three methods, REAG-B proved to be the most discriminatory method to differentiate isolates. The finding of genetically similar strains from the oral and tracheo-bronchial sites from the same patient supports the notion that the oral cavity may serve as an important source for C. albicans spread to the trachea and lung of mechanically ventilated patients.