Evidence for short-patch mismatch repair in Saccharomyces cerevisiae
Evidence for short-patch mismatch repair in Saccharomyces cerevisiae
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DOI：
10.1093/emboj/19.13.3408
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发表时间：
2000-07-03
期刊：
影响因子：
11.4
通讯作者：
Fabre, F
中科院分区：
文献类型：
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作者：
Coïc, E;Gluck, L;Fabre, F
不相同序列之间的双链化事件最常涉及进行错配修复的异源双链DNA中间体。在真核生物中由细菌MutS和MutL直系同源物控制的充分表征的长补丁错配修复过程是参与错配碱基修复的主要系统。在这里，我们提出了一个替代的短补丁错配修复途径，对广泛的错配的证据。在缺少长补丁修复系统的msh2突变体中，对相似但不相同的亲本DNA之间交换产生的重组片段进行序列分析，结果表明发生了短补丁修复事件，这可能涉及到
Recombination events between non-identical sequences most often involve heteroduplex DNA intermediates that are subjected to mismatch repair. The well-characterized long-patch mismatch repair process, controlled in eukaryotes by bacterial MutS and MutL orthologs, is the major system involved in repair of mispaired bases. Here we present evidence for an alternative short-patch mismatch repair pathway that operates on a broad spectrum of mismatches. In msh2 mutants lacking the long-patch repair system, sequence analysis of recombination tracts resulting from exchanges between similar but non-identical (homeologous) parental DNAs showed the occurrence of short-patch repair events that can involve