Down-regulation of statin, a nonproliferation-specific nuclear protein, and up-regulation of c-myc after initiation of programmed cell death in mouse fibroblasts.
Down-regulation of statin, a nonproliferation-specific nuclear protein, and up-regulation of c-myc after initiation of programmed cell death in mouse fibroblasts.
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小鼠成纤维细胞程序性细胞死亡启动后，他汀类药物（一种非增殖特异性核蛋白）下调，c-myc 上调。
DOI：
10.1002/jcp.1041630118
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Pandey,S
中科院分区：
文献类型：
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作者：
Wang,E;Pandey,S
生长因子的去活化已显示在许多细胞类型中诱导程序性细胞死亡，包括小鼠3 T3成纤维细胞。程序性细胞死亡（细胞凋亡）是一种自我毁灭的主动过程，被认为需要独特基因的表达。最近，细胞周期基因如c-fosandc-myc的表达，以及重新进入细胞周期遍历，被认为是诱导程序性细胞死亡所必需的。本实验室以前的工作表明，他汀类药物是一种非增殖特异性核蛋白，存在于年轻静止或衰老的人成纤维细胞的细胞核中，以及生长停滞的小鼠3 T3成纤维细胞中;我们已经报告说，他汀类药物在生长停滞的阻断被移除后迅速消失，细胞被允许恢复细胞周期遍历。在这份报告中，我们解决的问题，是否细胞诱导进入程序性细胞死亡过程也失去了他汀类药物的表达。我们研究了密度停滞的静止小鼠3 T3细胞，其在血清剥夺后通过细胞凋亡经历快速细胞死亡。我们的研究结果表明，c-mycexpression是诱导的，如以前在其他系统的凋亡死亡。有趣的是，我们还发现，他汀类药物确实消失后，程序性细胞死亡的诱导启动。这些结果进一步支持了这样的观点，即当诱导凋亡时，细胞表现得好像从复制停滞中释放出来，并经历细胞周期的G1期的一部分。此事件与正常细胞周期遍历之间的差异在于，凋亡过程中的G1期经历是失败的，最终结果是细胞死亡。© 1995 Wiley利斯公司
Deprivation of growth factors has been shown to induce programmed cell death in many cell types, including mouse 3T3 fibroblasts. Programmed cell death (apoptosis) is an active process of self‐destruction which is thought to require the expression of unique genes. Recently, the expression of cell cycle genes such asc‐fosandc‐myc, and re‐entrance to cell cycle traverse, are thought to be necessary to induce programmed cell death. Previous work in this laboratory has shown that statin is a nonproliferation‐specific nuclear protein present in the nuclei of young quiescent or senescent human fibroblasts, as well as in growth‐arrested mouse 3T3 fibroblasts; we have reported that statin disappears rapidly after the blockage of growth arrest is removed and cells are allowed to resume cell cycle traverse. In this report we address the question of whether cells induced to enter the programmed cell death process also lose the expression of statin. We studied density‐arrested quiescent mouse 3T3 cells, which undergo rapid cell death by apoptosis upon serum deprivation. Our results suggest thatc‐mycexpression is induced, as previously reported in other systems of apoptotic death. Interestingly, we also find that statin indeed disappears after the induction of programmed cell death is initiated. These results further support the notion that when apoptosis is induced, cells behave as though released from replication arrest, and experience some part of the G1phase of the cell cycle. The difference between this event and normal cell cycle traverse is that this experience of the G1phase in the apoptotic process is an abortive one, with the end result of cell demise. © 1995 Wiley‐Liss, Inc.