Ontogenetic localization and distribution of S-100β protein in human placental tissues
Ontogenetic localization and distribution of S-100β protein in human placental tissues
复制标题
DOI：
10.1016/s0029-7844(02)01996-8
复制
发表时间：
2002-06-01
影响因子：
7.2
通讯作者：
Cosmi, EV
中科院分区：
文献类型：
--
作者：
Marinoni, E;Di Iorio, R;Cosmi, EV
目的：研究S-100 β，一种在羊水和胎儿循环中发现的脑特异性蛋白，是否存在于整个妊娠期间的胎儿胎盘组织中。结果：S-100 β蛋白在胎盘组织中主要定位于绒毛和中间滋养层细胞。免疫染色强度和蛋白质浓度随妊娠的进展而增加。S-100 β蛋白也存在于羊膜，滋养层，蜕膜细胞的胎膜，并在脐血管内皮细胞在所有孕龄。结论：这项研究表明，胎儿胎盘组织中含有S-100 β蛋白，这表明这些组织可能，至少部分，是负责高水平的胎儿循环中发现。虽然胎盘S-100 β的意义尚不清楚，但当这种蛋白质被用作胎儿或婴儿出生时脑损伤的标志物时，应考虑到这一来源。（妇产科2002;99：1093-9。(C)2002年由美国妇产科医师学会出版）。
OBJECTIVE: To investigate whether S-100beta, a brain-specific protein found in amniotic fluid and fetal circulation, is present in fetoplacental tissues throughout gestation.METHODS: S-100beta protein localization and concentration were assessed in placentae, fetal membranes, and cord vessels. Tissues were obtained from 40 pregnant women at different gestational ages: first trimester (n = 10), second trimester (n = 10), early third trimester (n = 10), and late third trimester (n = 10).RESULTS: In the placenta, S-100beta was localized in villous and intermediate trophoblast cells. The intensity of immunostaining and protein concentration increased with advancing gestation. S-100beta protein was also present in amnion, trophoblast, and decidual cells of fetal membranes, and in endothelial cells of umbilical vessels at all gestational ages.CONCLUSION: This study demonstrated that fetoplacental tissues contain S-100beta protein, suggesting that these tissues may, at least in part, be responsible for the high level found in the fetal circulation. Although the significance of placental S-100beta is unknown, this origin should be taken into account when this protein is used as a marker of brain injury in the fetus or infant at birth. (Obstet Gynecol 2002;99:1093-9. (C) 2002 by the American College of Obstetricians and Gynecologists).