Statistical optimization of xylanase production by Aspergillus niger AN-13 under submerged fermentation using response surface methodology
Statistical optimization of xylanase production by Aspergillus niger AN-13 under submerged fermentation using response surface methodology
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DOI：
10.4314/ajb.v7i5.58488
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发表时间：
2008
影响因子：
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通讯作者：
Yu Cao;De-jing Meng;Jian Lu;Jie Long
中科院分区：
文献类型：
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作者：
Yu Cao;De-jing Meng;Jian Lu;Jie Long
采用响应面法（RSM）评估了培养时间、pH值和底物浓度对黑曲霉AN - 13产木聚糖酶的影响。在深层发酵条件下，以农业废弃物麦麸作为主要底物。通过Box - Behnken设计（BBD）对木聚糖酶的生产进行了优化。结果的统计分析表明，这三个变量的一次项和二次项具有显著影响，并且发现pH值和底物浓度之间存在明显的交互作用，在显著水平上对响应有贡献。此外，用于处理组合分析的Box - Behnken设计（BBD）给出了一个二阶多项式回归模型，该模型与实验结果吻合良好，R² = 0.9959（P <0.0001）。通过对回归模型的分析，确定了最佳条件，即培养时间为53.3小时，pH值为7.92，麦麸浓度为54.2 g·L⁻¹，该模型预测木聚糖酶活性为125.14 U·mL⁻¹。优化验证表明，在最佳条件下观察到木聚糖酶产量为127.12 U·mL⁻¹，与Box - Behnken设计实验中的木聚糖酶活性4.80 U·mL⁻¹相比有显著提高。
Response surface methodology (RSM) was performed to evaluate the effects of cultivation time, pH and substrate concentration on production of xylanase by Aspergillus niger AN-13. Agricultural residue wheat bran was used as main substrate under submerged fermentation. Xylanase production was optimized by Box-Behnken design (BBD). Statistical analysis of results showed that, the linear and quadric terms of these three variables had significant effects, and evident interactions existing between pH and substrate concentration were found to contribute to the response at a significant level. Furthermore, Box-Behnken design (BBD) used for the analysis of treatment combinations gave a second-order polynomial regression model, which was in good agreement with experimental results, with R2=0.9959 (P namely cultivation time of 53.3 h, pH of 7.92 and wheat bran concentration of 54.2 g·L-1, the model predicted a xylanase activity of 125.14 U·mL-1. Verification of the optimization showed that xylanase production of 127.12 U·mL-1 was observed under the optimal condition, which had a marked increase compared with a xylanase activity of 4.80 U·mL-1 in experiments according to Box-Behnken design.