Photonics - Biomedical Photonics, Spectroscopy, and Microscopy
Photonics - Biomedical Photonics, Spectroscopy, and Microscopy
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光子学 - 生物医学光子学、光谱学和显微镜
DOI：
10.1002/9781119011804.ch1
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Birch D
中科院分区：
文献类型：
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作者：
Birch D
本章概述了通过荧光实现的一些主要功能、技术和测量。它涵盖了光谱、量子产额、寿命、猝灭、各向异性和显微镜，在每个案例中都引用了专题综述文章、许多原始参考文献、基本理论和现代应用。在任何荧光研究中，测量吸收光谱和荧光光谱通常是第一步。为了说明吸收光谱和荧光光谱通常是如何相互作用的，本章考虑了3，4-二羟基-L-苯丙氨酸(L-多巴)自动氧化产生黑色素的例子。寿命测量是近年来出现的荧光光谱学中功能最强大、用途最广的技术。荧光各向异性技术的基础是使用偏振激发来创建激发的荧光分子的空间选择的、非随机的分布，然后随机化，最常见的是通过布朗旋转，但有时也通过取决于系统的能量迁移。
This chapter surveys some of the main capabilities, techniques, and measurements that are enabled by fluorescence. It covers spectra, quantum yield, lifetime, quenching, anisotropy, and microscopy, in each case citing topical review articles, many of the original references, underlying theory and modern day applications. Measuring absorption and fluorescence spectra is usually the first place to start in any fluorescence study. In order to illustrate how absorption and fluorescence spectra often interplay in tandem, the chapter considers the example of the auto‐oxidation of 3,4‐dihydroxy‐l‐phenylalanine (l‐DOPA) to produce melanin. Lifetime measurement has emerged in recent years as the most powerful and versatile technique in fluorescence spectroscopy. The basis of fluorescence anisotropy techniques is to use polarized excitation to create a spatially selected, non‐random, distribution of excited fluorescent molecules which then randomize, most commonly by Brownian rotation, but also at times by energy migration depending on the system.