DORSAL-ROOT GANGLION-CELL DEATH AND SURVIVING CELL NUMBERS IN RELATION TO THE DEVELOPMENT OF SENSORY INNERVATION IN THE RAT HINDLIMB
DORSAL-ROOT GANGLION-CELL DEATH AND SURVIVING CELL NUMBERS IN RELATION TO THE DEVELOPMENT OF SENSORY INNERVATION IN THE RAT HINDLIMB
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DOI：
10.1016/0165-3806(94)90163-5
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发表时间：
1994-10-14
期刊：
影响因子：
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通讯作者：
FITZGERALD, M
中科院分区：
文献类型：
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作者：
COGGESHALL, RE;POWER, CM;FITZGERALD, M
本研究将死亡、存活和增殖的L(4)初级传入神经元的数量与大鼠后肢周围感觉神经的发育联系起来。细胞死亡发生在胚胎第15天(E15)到出生后不久，在E17-E19达到高峰。尽管如此，存活的细胞数量在出生前稳步上升，这表明细胞死亡在这段时间内被细胞增殖所平衡。GAP-43免疫组织化学染色显示，胎龄15岁时，L4神经节的外周感觉轴突仅在后肢中央，直到出生时才到达其远端的外周靶点，因此L4神经节细胞的产前死亡与这些初级感觉神经轴突的周围神经支配的发育没有很好的相关性。然而，出生前的细胞死亡与中枢感觉轴突对脊髓的神经支配有很好的相关性。与存活细胞数量从胎龄15天到出生后5天的稳步上升相反，从出生到出生5天，存活细胞数量下降了16%。这种减少与周围神经的发育有关。我们的结论是，大鼠背根神经节的大部分细胞死亡是由局部或中枢因素控制的，而外周靶点因素可能在出生后发挥影响，决定哺乳动物感觉神经元的最终数量。数据还表明，细胞死亡可能有两个阶段，早期涉及大的亮细胞，晚期涉及小的暗细胞。
This study correlates the numbers of dying, surviving and proliferating L(4) primary afferent neurons with the development of peripheral hindlimb sensory innervation in the rat. Cell death occurs from embryonic day 15 (E15) to just after birth and peaks at E17-E19. Despite this, surviving cell numbers rise steadily to birth indicating that cell death is more than balanced by cell proliferation over this period. GAP-43 immunostaining indicates that the peripheral sensory axons are only in central parts of the hindlimb by E15 and do not finish arriving at their distal peripheral targets until birth so prenatal cell death in the L4 ganglion is not well correlated with the development of the peripheral innervation by these primary sensory axons. Prenatal cell death does, however, correlate well with the innervation of the cord by central sensory axons. In contrast to the steady rise of surviving cell numbers from E15 to birth, cell numbers go down 16% in the period from birth to postnatal day 5. This loss is correlated with the development of the peripheral innervation. We conclude that the bulk of cell death in the rat LP, dorsal root ganglion, which is prenatal, is controlled by local or central factors whereas peripheral target factors may exert their influence postnatally to determine the final numbers of mammalian sensory neurons. The data also suggest that there may be two phases of cell death, an early phase involving large light cells and a late phase involving small dark cells.