Levels of oxidative stress markers: correlation with hepatic function and worm burden patients with schistosomiasis
Levels of oxidative stress markers: correlation with hepatic function and worm burden patients with schistosomiasis
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DOI：
10.2478/s11686-012-0026-5
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发表时间：
2012-06-01
影响因子：
1.5
通讯作者：
Freire, A. C. G.
中科院分区：
文献类型：
--
作者：
Cunha, G. M. M.;Silva, V. M. A.;Freire, A. C. G.
血吸虫病是由巴西的公共卫生问题引起的。典型的肉芽肿性病变与自由基产生引起的氧化损伤增加有关。本研究的目的是将急性期血吸虫病携带者（n = 30）与健康受试者（n = 30）的一些氧化应激标志物与蠕虫负荷相关联。所用的促氧化剂参数是硫代巴比妥酸反应物质的比色定量，而所用的抗氧化剂标记物是还原型谷胱甘肽的血液含量和过氧化氢酶活性的测定。采用Kato-Katz法计算虫负荷。结果指出，在开始时，过氧化氢酶活性没有差异。但寄生虫负荷的增加与脂质过氧化反应的强度和还原型谷胱甘肽含量的降低呈正相关。此外，仅天冬氨酸氨基转移酶水平升高，而胆红素水平降低。因此，在组织中的氧化应激的建立和寄生负载之间的可能的关联的建议。
Schistosomiasis is caused by and is a public health problem in Brazil. The typical granulomatous lesion is associated with the increase in the oxidative damage by generation of free radicals. The aim of this work was to correlate some oxidative stress markers with the worm burden on carriers of schistosomiasis (n = 30) in the acute phase in comparison to healthy subjects (n = 30). The pro-oxidant parameter used was the colorimetric quantification of reactive substances to thiobarbituric acid, while the antioxidant markers used were blood content of reduced glutathione and determination of the activity of catalase. The worm burden was assessed by Kato-Katz method. The results pointed out that initially there was no difference in the catalase activity. However, there was a positive correlation between the increase in parasitic load and intensity of lipid peroxidation, and decrease in the content of reduced glutathione. Additionally, only the aspartate aminotransferase levels presented to be high, while there was a decrease in bilirubin level. Therefore, a possible association between the establishment of the oxidative stress in tissue and the parasitic load of is suggested.