BROMODEOXYURIDINE MUTAGENESIS IN MAMMALIAN-CELLS - MUTAGENESIS IS INDEPENDENT OF AMOUNT OF BROMOURACIL IN DNA
BROMODEOXYURIDINE MUTAGENESIS IN MAMMALIAN-CELLS - MUTAGENESIS IS INDEPENDENT OF AMOUNT OF BROMOURACIL IN DNA
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DOI：
10.1073/pnas.75.10.4982
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
DAVIDSON, RL
中科院分区：
文献类型：
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作者：
KAUFMAN, ER;DAVIDSON, RL
当核DNA中的所有胸腺嘧啶残基被溴尿嘧啶（BrUra）取代时，突变的叙利亚仓鼠黑色素瘤细胞（HAB-2 E）系显示出无限的生长潜力，如何避免溴脱氧尿苷（BrdUrd）致突变性的有害影响。HAB-2 E细胞DNA中BrUra含量与其致突变性呈非线性关系。对于这些细胞，致突变性显然是由细胞暴露的BrdUrd浓度决定的，而不是DNA中BrUra的量。这些结果是以哇巴因抗性和硫鸟嘌呤抗性的诱导作为诱变标记而获得的。在亲本黑色素瘤细胞中观察到BrdUrd致突变性对BrdUrd浓度的依赖性。
How a line of mutant Syrian hamster melanoma cells (HAB-2E) that displays unlimited growth potential when all of the thymine residues in nuclear DNA are replaced by bromouracil (BrUra) could avoid the deleterious effects of bromodeoxyuridine (BrdUrd) mutagenicity was determined. There was a nonlinear relationship between mutagenicity and the amount of BrUra in the DNA of the HAB-2E cells. With these cells, mutagenicity apparently was determined by the concentration of BrdUrd to which the cells were exposed rather than the amount of BrUra in DNA. These results were obtained with the induction of ouabain resistance and thioguanine resistance as markers for mutagenesis. The dependence of BrdUrd mutagenicity on BrdUrd concentration was observed for the parental melanoma cells.