Regulation of canine renal vesicle Pi transport by growth hormone and parathyroid hormone.
Regulation of canine renal vesicle Pi transport by growth hormone and parathyroid hormone.
复制标题
生长激素和甲状旁腺激素对犬肾小泡 Pi 转运的调节。
DOI：
10.1016/0005-2736(80)90378-8
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Hruska,KA
中科院分区：
文献类型：
--
作者：
Hammerman,MR;Karl,IE;Hruska,KA
生长激素（GH）可增加肾磷酸盐（Pi）重吸收，甲状旁腺激素（PTH）可减少肾磷酸盐（Pi）重吸收. Na+刺激的Pi跨近曲小管刷状缘膜转运是Pi重吸收过程的起始步骤。为了确定由GH或PTH引起的Pi重吸收的变化是否伴随着刷状缘膜Na+梯度刺激的Pi转运的变化，我们通过从犬肾制备的分离刷状缘膜囊泡检测了体内GH和PTH给药以及甲状腺甲状旁腺切除术对Pi转运的影响。在检查PTH给药效果的实验中，同一动物提供对照肾（PTH给药前）和实验肾（PTH给药后）。在体内PTH给药后，从正常、GH治疗和甲状腺甲状旁腺切除犬分离的囊泡中的Na+-梯度P i过冲增加。生长激素管理和甲状腺甲状旁腺切除术增加了高度的超调相比，正常。甲状旁腺素给药降低了44%，从正常动物的囊泡的表观V值。与正常值相比，GH（34%）和甲状旁腺切除术（57%）的表观V值增加。PTH给药降低了后两组的表观V。GH给药的甲状腺甲状旁腺切除犬进一步增加了明显的V。在P i重吸收在体内的实验操作引起的明显V的变化。我们的结论是，在肾脏P i重吸收的变化由GH引起的类似于由PTH引起的，伴随着在肾刷状缘膜中的Na+刺激的P i转运系统的变化，并且PTH对囊泡的P i转运的影响在GH治疗的狗中与对来自正常动物的囊泡的影响没有区别。
Renal phosphate (P i) reabsorption is increased by growth hormone (GH) and decreased by parathyroid hormone (PTH). Na+-stimulated P i transport across the brush border membrane of the proximal tubule is the initial step in the process of P i reabsorption. To determine whether changes in P i reabsorption induced by GH or PTH are accompanied by changes in brush border membrane Na+-gradient-stimulated P i transport, we examined the effect of in vivo GH and PTH administration and thyroparathyroidectomy on P i transport by isolated brush border membrane vesicles prepared from canine kidney. In experiments in which the effect of PTH administration was examined, the same animal provided the control kidney (before PTH administration) and the experimental kidney (after PTH administration). The Na+-gradient P i overshoot in vesicles isolated from normal, GH-treated and thyroparathyroidectomized dogs was increased after in vivo PTH administration. GH administration and thyroparathyroidectomy increased the height of the overshoot compared to normal. PTH administration decreased the apparent V value by 44% in vesicles from normal animals. The apparent V value was increased, compared to normal, by GH (34%) and thyroparathyroidectomy (57%). PTH administration decreased the apparent V in both the latter groups. GH administration to thyroparathyroidectomized dogs further increased the apparent V. Changes in the apparent V paralleled changes in P i reabsorption in vivo induced by experimental manipulations. We conclude that changes in renal P i reabsorption induced by GH were like those induced by PTH, accompanied by changes in the Na+-stimulated P i transport system in the renal brush border membrane, and that the effect of PTH on vesicular P i transport in GH-treated dogs did not differ from the effect on vesicles from normal animals.