A pneumococcal capsular polysaccharide vaccine induces a repertoire shift with increased VH3 expression in peripheral B cells from human immunodeficiency virus (HIV)-uninfected but not HIV-infected persons
A pneumococcal capsular polysaccharide vaccine induces a repertoire shift with increased VH3 expression in peripheral B cells from human immunodeficiency virus (HIV)-uninfected but not HIV-infected persons
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DOI：
10.1086/315405
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发表时间：
2000-04-01
影响因子：
6.4
通讯作者：
Pirofski, L
中科院分区：
文献类型：
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作者：
Chang, Q;Abadi, J;Pirofski, L
人类免疫缺陷病毒（HIV）感染者肺炎球菌疫苗失败的分子机制尚未完全了解。采用聚合酶链反应ELISA法测定12名HIV感染者和12名HIV未感染者在肺炎球菌疫苗接种前和接种后7天外周血IgG、伊加和IgM CD 19阳性B细胞表达6个免疫球蛋白重链可变区（V-H）亚群的比例。在未感染HIV的组中，观察到免疫后IgG和伊加的V（H）3亚群表达显著增加，以及对免疫的更大血清学应答。相反，HIV感染组在接种前IgG V（H）3降低，而在接种后IgG V（H）5增加。这些结果表明，肺炎球菌疫苗接种改变了B细胞V-H基因表达的模式，并支持了这样的概念，即异常的V（H）3表达可能转化为HIV感染背景下的不良抗肺炎球菌反应。
The molecular mechanism of pneumococcal vaccine failure in human immunodeficiency virus (HIV)-infected persons is not fully understood. A polymerase chain reaction ELISA was used to determine the proportion of peripheral IgG, IgA, and IgM CD19-positive B cells expressing 6 immunoglobulin heavy-chain variable region (V-H) subgroups before and 7 days after pneumococcal vaccination of 12 HIV-infected and 12 HIV-uninfected subjects. Significant postvaccination increases in the expression of the V(H)3 subgroup by IgG and IgA and a greater serologic response to vaccination were observed in the HIV-uninfected group. In contrast, the HIV-infected group had reduced prevaccination IgG V(H)3 and a postvaccination increase in IgG V(H)5. These results demonstrate that pneumococcal vaccination changes the pattern of B cell V-H gene expression and support the concept that aberrant V(H)3 expression may translate into a poor antipneumococcal response in the setting of HIV infection.