No evidence of correlation between mutation at codon 531 of src and the risk of colon cancer in Chinese
No evidence of correlation between mutation at codon 531 of src and the risk of colon cancer in Chinese
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DOI：
10.1016/s0304-3835(99)00398-5
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发表时间：
2000-03-31
期刊：
影响因子：
9.7
通讯作者：
Chang, JG
中科院分区：
文献类型：
--
作者：
Wang, NM;Yeh, KT;Chang, JG
c-src原癌基因产物pp 60（c-src）的蛋白酪氨酸激酶活性在许多人类癌症（包括结肠癌）中升高。人pp 60（c-src）羧基端酪氨酸530的磷酸化抑制其激酶活性。最近有报道认为src基因密码子531（Gln-531 --> Amber-531）发生C --> T转换突变的人患结肠癌的风险更高。该突变导致翻译提前终止并上调激酶活性。为了研究这种突变是否可能是中国人群中结肠癌的危险因素，我们使用相同的基于PCR的检测方法分析了131例结肠癌和其他各种类型的癌症的src基因型。在本研究筛选的所有标本中均未检测到突变。因此，src基因Gln-531位点突变与中国人结肠癌的发生无关。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
The protein tyrosine kinase activity of c-src proto-oncogene product, pp60(c-src), is elevated in a number of human cancers, including colon cancer. Phosphorylation of human pp60(c-src) carboxy-terminal tyrosine 530 suppresses its kinase activity. A recent report suggested that the risk of colon cancer is higher for those who carry a C --> T transition mutation on codon 531 (Gln-531 --> Amber-531) of src gene. This mutation caused a prematured translation termination and up-regulated the kinase activity. To examine whether this mutation could be a risk factor for colon carcinoma in the Chinese population, we used the same PCR-based assay to analyze src genotypes of 131 colon cancers and other various types of carcinoma. No mutation was detected in all specimens that were screened in this study. Thus, mutation at Gln-531 of src gene does not seem to be involved in the development of colon cancer in Chinese ethnicity. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.