Primary recovery of bioactive compounds from stressed carrot tissue using aqueous two-phase systems strategies
Primary recovery of bioactive compounds from stressed carrot tissue using aqueous two-phase systems strategies
复制标题
使用水性两相系统策略从应激胡萝卜组织中初步回收生物活性化合物
DOI：
10.1002/jctb.4553
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Jorge Benavides
中科院分区：
文献类型：
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作者：
Juan Carlos Sánchez;D. A. Jacobo;L. Cisneros;Jorge Benavides
背景
胡萝卜采后非生物胁迫诱导产生的酚类化合物，主要是绿原酸，这是目前不同的生物功能。双水相系统（ATPS）是一种液-液萃取技术，已被证明具有分离和回收生物活性化合物的潜力。ATPS具有使用环境友好成分、易于放大和过程集成能力的优点。这项实验工作解决了第一次的回收生物活性化合物（主要是羟基肉桂酸衍生物）从非生物胁迫胡萝卜组织使用ATPS策略，包括原位提取，在醇-盐和离子液体-盐系统。
结果
乙醇-磷酸钾体系有利于绿原酸的分配，回收率为81.9%。羟基肉桂酸衍生物在1-乙基-3-甲基咪唑乙酸盐（EMIM-Ac）-磷酸钾ATPS体系中得到了较好的分离，回收率可达85.4%。原位提取策略不仅表现出潜在的合并提取和回收在同一系统中，但也取决于ATPS使用，允许差分酚类配置文件获得。
结论
ATPS策略（固液提取后ATPS或原位提取）被证明具有很大的潜力，从应激胡萝卜组织的生物活性物质的回收。© 2014化学工业协会
BACKGROUND
The application of controlled postharvest abiotic stresses in carrots induces the production of phenolic compounds, mainly chlorogenic acids, which present diverse biological functions. Aqueous two-phase systems (ATPS) is a liquid–liquid extraction technique that has demonstrated its potential for the fractionation and recovery of bioactive compounds. ATPS have the advantages of using environmentally friendly constituents, ease of scale-up and process integration capability. This experimental work addresses for the first time the recovery of bioactive compounds (mainly hydroxycinnamic acid derivatives) from abiotically stressed carrot tissue using ATPS strategies, including in situ extraction, in alcohol–salt and ionic liquid–salt systems.
RESULTS
The partitioning of chlorogenic acid was favored in the ethanol–potassium phosphate system achieving a recovery yield of 81.9%. Hydroxycinnamic acid derivatives fractioning was favored in 1-ethyl-3-methylimidazolium acetate (EMIM-Ac)–potassium phosphate ATPS, where recovery yields up to 85.4% were achieved. The in situ extraction strategy not only demonstrated potential for merging extraction and recovery in the same system but also, depending on ATPS used, allowed differential phenolic profiles to be obtained.
CONCLUSION
ATPS strategies (either solid–liquid extraction followed by ATPS or in situ extraction) were shown to have great potential for the recovery of bioactives from stressed carrot tissue. © 2014 Society of Chemical Industry