Distinct Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Older Adults With Frailty.
Distinct Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Older Adults With Frailty.
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不同的血清和粪便代谢物谱与虚弱老年人的肠道微生物组相关。
DOI：
10.3389/fmed.2022.827174
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发表时间：
2022
影响因子：
3.9
通讯作者：
中科院分区：
文献类型：
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作者：
虚弱是一种重要的衰老相关综合征，但其潜在的代谢机制仍知之甚少。本研究的目的是基于代谢组学和肠道微生物组学的综合分析来鉴定新的生物标志物并揭示虚弱的潜在机制。在这项研究中，20名受试者包括5名中年人和15名老年人，其中15名老年受试者被分为三组：非虚弱、虚弱前期和虚弱，每组5名受试者。根据身体虚弱表型（PFP）确定虚弱、虚弱前期或非虚弱的存在。我们将非靶向代谢组学应用于血清和粪便样本，并使用16 S rDNA基因测序来检测粪便微生物组。通过斯皮尔曼相关分析分析代谢物和肠道微生物群之间的关联。虚弱时的血清代谢变化主要包括脂肪酸及其衍生物、碳水化合物和单糖。大多数属于这些类别的代谢物在虚弱的老年人的血清中增加。对羟基苯甲酸丙酯被发现在非虚弱，虚弱前，虚弱的老年人逐渐减少。在中年成人、非虚弱和虚弱的老年受试者中也发现了粪便代谢物谱和肠道微生物群的明显变化。脆弱区粪杆菌属、罗斯拜瑞氏菌属和梭形菌属的相对丰度下降，而副拟杆菌属和拟杆菌属的相对丰度增加。上述微生物的改变与虚弱时血清代谢物的改变有关，包括十二烷二酸、D-核糖、D-（-）-甘露醇、肌酸和吲哚及其相关粪便代谢物。这些变化的微生物群和相关代谢产物可作为机体虚弱的生物标志物，值得进一步研究。
Frailty is a critical aging-related syndrome but the underlying metabolic mechanism remains poorly understood. The aim of this study was to identify novel biomarkers and reveal potential mechanisms of frailty based on the integrated analysis of metabolome and gut microbiome. In this study, twenty subjects consisted of five middle-aged adults and fifteen older adults, of which fifteen older subjects were divided into three groups: non-frail, pre-frail, and frail, with five subjects in each group. The presence of frailty, pre-frailty, or non-frailty was established according to the physical frailty phenotype (PFP). We applied non-targeted metabolomics to serum and feces samples and used 16S rDNA gene sequencing to detect the fecal microbiome. The associations between metabolites and gut microbiota were analyzed by the Spearman’s correlation analysis. Serum metabolic shifts in frailty mainly included fatty acids and derivatives, carbohydrates, and monosaccharides. Most of the metabolites belonging to these classes increased in the serum of frail older adults. Propylparaben was found to gradually decrease in non-frail, pre-frail, and frail older adults. Distinct changes in fecal metabolite profiles and gut microbiota were also found among middle-aged adults, non-frail and frail older subjects. The relative abundance of Faecalibacteriu, Roseburia, and Fusicatenibacter decreased while the abundance of Parabacteroides and Bacteroides increased in frailty. The above altered microbes were associated with the changed serum metabolites in frailty, which included dodecanedioic acid, D-ribose, D-(-)-mannitol, creatine and indole, and their related fecal metabolites. The changed microbiome and related metabolites may be used as the biomarkers of frailty and is worthy of further mechanistic studies.