Caveolin1 Tyrosine-14 Phosphorylation: Role in Cellular Responsiveness to Mechanical Cues
Caveolin1 Tyrosine-14 Phosphorylation: Role in Cellular Responsiveness to Mechanical Cues
复制标题
DOI：
10.1007/s00232-020-00143-0
复制
发表时间：
2020-10-22
影响因子：
2.4
通讯作者：
Balasubramanian, Nagaraj
中科院分区：
文献类型：
--
作者：
Buwa, Natasha;Mazumdar, Debasmita;Balasubramanian, Nagaraj
质膜是一个动态的脂质双层，与细胞外微环境和细胞内细胞骨架结合。小凹是独特的质膜内陷，由整合膜蛋白 Caveolin1、2 和 3 排列。小凹的形成和稳定性得到其他蛋白质（包括 Cavin1、EHD2、Pacsin2 和 ROR1）的进一步支持。小凹膜的脂质成分富含胆固醇和磷脂酰丝氨酸，积极促进小凹的形成和功能。 Cav1 的翻译后修饰，包括酪氨酸 14 残基 (pY14Cav1) 的磷酸化，对其进出小凹的功能至关重要。经受显着机械应力的细胞具有丰富的小凹。它们在调节细胞信号传导和内吞作用中发挥着至关重要的作用，这可能会进一步影响 PM 处小凹的丰度和分布，有助于传感和/或缓冲机械应力。细胞响应多种机械刺激的膜张力变化会影响小凹的组织和功能。这些机械信号调节 pY14Cav1 水平以及在小凹和粘着斑中的功能。这篇综述除了研究小窝的机械敏感性性质外，还重点关注 pY14Cav1 在调节细胞机械转导中的作用。[图表]。
The plasma membrane is a dynamic lipid bilayer that engages with the extracellular microenvironment and intracellular cytoskeleton. Caveolae are distinct plasma membrane invaginations lined by integral membrane proteins Caveolin1, 2, and 3. Caveolae formation and stability is further supported by additional proteins including Cavin1, EHD2, Pacsin2 and ROR1. The lipid composition of caveolar membranes, rich in cholesterol and phosphatidylserine, actively contributes to caveolae formation and function. Post-translational modifications of Cav1, including its phosphorylation of the tyrosine-14 residue (pY14Cav1) are vital to its function in and out of caveolae. Cells that experience significant mechanical stress are seen to have abundant caveolae. They play a vital role in regulating cellular signaling and endocytosis, which could further affect the abundance and distribution of caveolae at the PM, contributing to sensing and/or buffering mechanical stress. Changes in membrane tension in cells responding to multiple mechanical stimuli affects the organization and function of caveolae. These mechanical cues regulate pY14Cav1 levels and function in caveolae and focal adhesions. This review, along with looking at the mechanosensitive nature of caveolae, focuses on the role of pY14Cav1 in regulating cellular mechanotransduction.[GRAPHICS].