Evaluation of Nanofiber-Based Polyglycolic Acid Scaffolds for Improved Chondrocyte Retention and In Vivo Bioengineered Cartilage Regeneration
Evaluation of Nanofiber-Based Polyglycolic Acid Scaffolds for Improved Chondrocyte Retention and In Vivo Bioengineered Cartilage Regeneration
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DOI：
10.1097/prs.0000000000000176
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发表时间：
2014-06-01
影响因子：
3.6
通讯作者：
Isogai, Noritaka
中科院分区：
文献类型：
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作者：
Itani, Yoshihito;Asamura, Shinichi;Isogai, Noritaka
背景资料：在使用大型动物的自体模型中进行的耳廓形软骨再生的常规研究中，存在软骨再生诱导能力和几何形状的长期保持的问题。在这项研究中，作者试图改善这些方面的结果：通过纳米技术开发了聚乙醇酸非织造织物，并评估了纤维直径对体外细胞接种效率的影响以及植入自体大动物模型后的体内反应。方法：分离犬软骨细胞并接种在平均直径为0.5至20 μ m的聚乙醇酸织物上。细胞接种效率是最高的中档聚乙醇酸纤维（平均直径，0.8，3.0，和7.0 μ m）。使用聚丙烯结构支撑物构建扁平和耳廓形支架，包裹含有自体软骨细胞以及碱性成纤维细胞生长因子负载颗粒和外部纤维蛋白密封剂的非织造聚乙醇酸织物。支架，然后植入自体和评价在5和20 weeks.Results：生物力学强度是最佳的聚乙醇酸纤维直径为0.8至3.0 μ m。最佳的细胞维护和新生软骨反应，看到与聚乙醇酸纤维直径在相同的中间范围内的tumor constructs.Conclusion：这些研究结果表明，在大型动物模型的耳廓形软骨再生的纳米级调制的潜力。
Background: In conventional studies on the regeneration of auricle-shaped cartilage in autogenous models using large animals, there were problems with the cartilage regeneration induction capacity and long-term retention of geometric shape. In this study, the authors sought to improve on outcome in these regards: a nonwoven fabric of polyglycolic acid was developed through nanotechnology, and the effect of nanofiber diameter on in vitro cell-seeding efficiency and the in vivo response after implantation in an autogenous large-animal model were evaluated.Methods: Canine chondrocytes were isolated and seeded onto polyglycolic acid fabric ranging from 0.5 to 20 mu m in average diameter. Cell seeding efficiency was highest for mid-range polyglycolic acid fibers (average diameter, 0.8, 3.0, and 7.0 mu m). Flat and auricle-shaped scaffolds were constructed using polypropylene structural support, sandwiching a nonwoven polyglycolic acid fabric that contained autogenous chondrocytes together with basic fibroblast growth factor-laden particles and an exterior fibrin sealant. Scaffolds were then implanted autogenously and evaluated at 5 and 20 weeks.Results: Biomechanical strength was optimal for polyglycolic acid fiber diameters of 0.8 to 3.0 mu m. Optimal cell maintenance and neocartilage response were seen with polyglycolic acid fiber diameters in the same mid-range for nanofiber constructs.Conclusion: These findings demonstrate the potential for nanoscale modulation of auricle-shaped cartilage regeneration in a large-animal model.