Highly sensitive detection of 6mA at single-base resolution based on A–C mismatch
Highly sensitive detection of 6mA at single-base resolution based on A–C mismatch
复制标题
基于 A–C 错配的单碱基分辨率下 6mA 的高灵敏度检测
作者：
Li Wei;Yang Hongmei;Wang Yafen;Weng Xiaocheng;Wang Fang
我们首次证明了基于DNA连接酶对A-C错配和6 mA-C错配的选择性连接反应，可以选择性地识别6 mA。该方法与RCA扩增相结合，在单碱基分辨率下实现了对dsDNA中6 mA的高灵敏度鉴定。
We first demonstrated that 6mA can be selectively recognized based on the selective ligation reaction of DNA ligase toward A–C mismatch and 6mA-C mismatch. This method, when further combined with amplification using RCA, achieved highly sensitive identification of 6mA in dsDNA at single-base resolution.