TRANSLATIONAL CONTROL OF MATERNAL GLP-1 MESSENGER-RNA ESTABLISHES AN ASYMMETRY IN THE C-ELEGANS EMBRYO
TRANSLATIONAL CONTROL OF MATERNAL GLP-1 MESSENGER-RNA ESTABLISHES AN ASYMMETRY IN THE C-ELEGANS EMBRYO
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DOI：
10.1016/0092-8674(94)90311-5
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发表时间：
1994-04-22
期刊：
影响因子：
64.5
通讯作者：
KIMBLE, J
中科院分区：
文献类型：
--
作者：
EVANS, TC;CRITTENDEN, SL;KIMBLE, J
In C.在线虫中，GLP-1基因编码一种膜受体，该受体是早期胚胎中前细胞命运所必需的。我们报告GLP-1蛋白集中于2- 28细胞胚胎的前卵裂球。相比之下，glp-1 mRNA存在于所有的卵裂球，直到8-细胞阶段。此外，glp-1 3'非翻译区可以限制报告mRNA翻译到前卵裂球。因此，母体glp-1 mRNA的翻译在C.线虫胚胎在果蝇胚胎中，母体glp-1 mRNA的调节与母体hunchback mRNA的调节有着惊人的相似之处。因此，在不同的生物体中胚胎不对称的建立可能涉及母体mRNA调控的保守机制。
In C. elegans, the glp-1 gene encodes a membrane receptor that is required for anterior cell fates in the early embryo. We report that GLP-1 protein is focalized to anterior blastomeres in 2- to 28-cell embryos. By contrast, glp-1 mRNA is present in all blastomeres until the 8-cell stage. Furthermore, the glp-1 3' untranslated region can restrict translation of a reporter mRNA to anterior blastomeres. Therefore, the translation of maternal glp-1 mRNA is temporally and spatially regulated in the C. elegans embryo. The regulation of maternal glp-1 mRNA has striking parallels to the regulation of maternal hunchback mRNA in the Drosophila embryo. Thus, the establishment of embryonic asymmetry in diverse organisms may involve conserved mechanisms of maternal mRNA regulation.