Cytotoxicity of indocyanine green on retinal pigment epithelium - Implications for macular hole surgery
Cytotoxicity of indocyanine green on retinal pigment epithelium - Implications for macular hole surgery
复制标题
DOI：
10.1001/archopht.121.10.1423
复制
发表时间：
2003-10-01
影响因子：
--
通讯作者：
Chen, HC
中科院分区：
文献类型：
--
作者：
Ho, JD;Tsai, RJF;Chen, HC
目的：目的探讨吲哚菁绿色（ICG）对培养的人视网膜色素上皮细胞（RPE）的细胞毒性作用及其对黄斑裂孔手术的影响。暴露持续时间范围为5分钟至3小时。结果：ICG浓度为5 mg/mL时，RPE细胞在孵育5 min内，形态无明显改变，脱氢酶活性无明显下降。当5 mg/mL ICG作用10 min、1 mg/mL ICG作用20 min或0.01 mg/mL ICG作用3 h时，细胞形态发生改变，线粒体脱氢酶活性下降，部分细胞坏死。结论：吲哚菁绿色对培养的人RPE细胞具有剂量和时间依赖性的细胞毒性。细胞死亡发生necross.Clinical Relevance：暴露的RPE细胞ICG浓度高达5毫克/毫升5分钟或更少的是无害的;长期暴露于低浓度的ICG是有毒的。由于ICG可能在玻璃体腔中保留很长一段时间，因此需要在黄斑裂孔手术期间彻底冲洗ICG。
Objective: To evaluate the potential cytotoxic effects of indocyanine green (ICG) on cultured human retinal pigment epithelium (RPE) and the resultant implications for macular hole surgery.Methods: Human RPE cells were exposed to ICG in concentrations from 0.001 to 5 mg/mL. The exposure duration ranged from 5 minutes to 3 hours. Light microscopy, MTS viability assay, and calcein AM-ethidium homodimer 1 staining were used to evaluate the cytotoxic effects of ICG.Results: The RPE cells incubated with up to 5 mg/mL of ICG for 5 minutes or less exhibited no morphologic change and no significant decrease in dehydrogenase activity. When RPE cells were exposed to 5 mg/mL of ICG for 10 minutes, 1 mg/mL of ICG for 20 minutes, or 0.01 mg/mL of ICG for 3 hours, cell morphologic features were altered, mitochondrial dehydrogenase activity decreased, and some cells were necrotic.Conclusions: Indocyanine green caused cytotoxicity in cultured human RPE in a dose- and time-dependent manner. Cell death occurred by necrosis.Clinical Relevance: Exposure of RPE cells to ICG concentrations up to 5 mg/mL for 5 minutes or less was not injurious; prolonged exposure to a low ICG concentration was toxic. Since ICG may be retained in the vitreous cavity for a lengthy period, thorough washout of ICG during macular hole surgery is required.