Global analysis of gene expression in renal ischemia-reperfusion in the mouse.
Global analysis of gene expression in renal ischemia-reperfusion in the mouse.
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DOI：
10.1006/bbrc.2002.6535
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发表时间：
2002-03
影响因子：
3.1
通讯作者：
Takumi Yoshida;S. Tang;L. Hsiao;R. Jensen;J. Ingelfinger;S. Gullans
中科院分区：
文献类型：
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作者：
Takumi Yoshida;S. Tang;L. Hsiao;R. Jensen;J. Ingelfinger;S. Gullans
缺血性急性肾功能衰竭（ARF）是一种相对常见的疾病，具有较高的发病率和死亡率。为了研究ARF过程中的整体基因表达，6周龄C57 BL/6雄性小鼠经历了30分钟的双侧肾缺血，然后再灌注[I/R]或假手术。使用具有约10，000个基因的寡核苷酸微阵列[Affyssin]，其中6，643个存在于小鼠肾脏中，用于分析I/R后长达4天的mRNA表达。在第1天有52个基因和第4天有40个基因上调超过4倍[400%]。第1天的70个基因和第4天的30个基因下调至基线的0.25倍以下[25%]。实时定量RT-PCR证实了8个感兴趣基因的表达变化。大多数诱导的转录物涉及细胞结构、细胞外基质、细胞内钙结合和细胞分裂/分化。我们的数据确定了几个新的基因，可能是重要的缺血后肾修复。
Ischemia-induced acute renal failure (ARF) is a relatively common disorder with major morbidity and mortality. To study global gene expression during ARF, 6-week-old C57BL/6 male mice underwent 30 min of bilateral renal ischemia followed by reperfusion [I/R] or sham operation. Oligonucleotide microarrays [Affymetrix] with approximately 10,000 genes, 6,643 of which were present in mouse kidney, were used to analyze mRNA expression for up to 4 days following I/R. Fifty-two genes at day 1 and 40 at day 4 were up-regulated more than 4-fold [400%]. Seventy genes at day 1 and 30 genes at day 4 were down-regulated to under 0.25-fold from baseline [25%]. Real-time quantitative RT-PCR confirmed changes in expression for 8 genes of interest. Most of the induced transcripts are involved in cell structure, extracellular matrix, intracellular calcium binding, and cell division/differentiation. Our data identified several novel genes that may be important in renal repair after ischemia.