Nontuberculous Mycobacteria Isolated from Tuberculosis Suspects in Ibadan, Nigeria.
Nontuberculous Mycobacteria Isolated from Tuberculosis Suspects in Ibadan, Nigeria.
复制标题
DOI：
10.1155/2016/6547363
复制
发表时间：
2016
影响因子：
2.6
通讯作者：
Taiwo B
中科院分区：
文献类型：
--
作者：
Cadmus SI;Diarra B;Traore B;Maiga M;Siddiqui S;Tounkara A;Falodun O;Lawal W;Adewole IF;Murphy R;van Soolingen D;Taiwo B
尼日利亚是结核病负担最重的国家之一，在直接监督短程治疗(DOTS)中心，痰涂片显微镜被常规用于结核病诊断。该诊断算法不区分结核分枝杆菌复合体(MTC)和非结核分枝杆菌(NTM)。2008年12月至2009年1月，尼日利亚伊巴丹的10个DOTS中心对连续被诊断为结核病的患者进行了筛查。为了验证确诊患者中的分枝杆菌种类，我们使用了聚合酶链式反应、线探针分析和分型技术对分枝杆菌分离株进行了培养和鉴定。在48名筛查的患者中，有23人符合研究的纳入标准。所有23名研究患者都有阳性培养。总体而言，我们确定了11/23(48%)仅患有MTC，9/23(39%)仅患有NTM，3/23(13%)同时存在MTC和NTM。已鉴定的MTC菌株为拉丁美洲地中海(LAM)型(n=12)，非洲分枝杆菌(M.African anum)(n=1)和T基因族(n=1)。四种禽类-细胞内分枝杆菌。鉴定出囊藻复合体、1个龟分枝杆菌复合体、1个脓肿分枝杆菌和1个胞内分枝杆菌。我们的发现强调了在DOTS中心纳入分子技术以更准确地诊断结核病的必要性，以改善临床结果并保护公共卫生，特别是在结核病流行国家。
In Nigeria, one of the highest tuberculosis (TB) burdened nations, sputum smear microscopy is routinely employed for TB diagnosis at Directly Observed Treatment Short-Course (DOTS) Centers. This diagnostic algorithm does not differentiate Mycobacterium tuberculosis complex (MTC) from nontuberculous mycobacteria (NTM). Between December 2008 and January 2009, consecutive patients diagnosed with TB were screened for inclusion at 10 DOTS centers in Ibadan, Nigeria. To verify Mycobacterium species in patients diagnosed, we cultured and identified mycobacterial isolates using PCR, line probe assay, and spoligotyping techniques. From 48 patients screened, 23 met the inclusion criteria for the study. All the 23 study patients had a positive culture. Overall, we identified 11/23 patients (48%) with MTC only, 9/23 (39%) with NTM only, and 3/23 (13%) with evidence of both MTC and NTM. Strains of MTC identified were Latin American Mediterranean (LAM) genotype (n = 12), M. africanum (n = 1), and the genotype family T (n = 1). Four M. avium-intracellulare-M. scrofulaceum complexes, one M. chelonae complex, one M. abscessus, and one M. intracellulare were identified. Our findings underscore the need to incorporate molecular techniques for more precise diagnosis of TB at DOTS centers to improve clinical outcomes and safe guard public health, particularly in TB endemic countries.