Sensitive and Selective Colorimetric Visualization of Cerebral Dopamine Based on Double Molecular Recognition
Sensitive and Selective Colorimetric Visualization of Cerebral Dopamine Based on Double Molecular Recognition
复制标题
基于双分子识别的大脑多巴胺的灵敏选择性比色可视化
DOI：
10.1002/anie.201007071
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发表时间：
2011-01-01
影响因子：
16.6
通讯作者：
Shi, Guoyue
中科院分区：
文献类型：
--
作者：
Kong, Biao;Zhu, Anwei;Shi, Guoyue
直接的，选择性的，和灵敏的检测方法的发展，涉及脑功能的生理重要物种引起了人们的广泛关注，因为分析方法仍然是瓶颈的进展，了解神经递质的作用，神经活动，动物行为，等等。[1]多巴胺（DA）是最重要的神经递质之一，因为它在大脑功能中起着核心作用，如奖励相关的行为，运动和情绪。[2]神经元释放和摄取DA的改变与精神疾病、成瘾和帕金森病有关。[3]在过去的30年里，大量优雅的方法，包括电化学策略、光谱方法、色谱法等，已被用于DA检测。[1c然而，大脑系统的复杂性提出了一个巨大的挑战，这些现有的方法，提供理论和技术上简单的DA检测在大脑system.There，我们首先报告了一个直接的，选择性的，和灵敏的策略，在纳摩尔水平上使用金纳米粒子（AuNPs）的大脑DA的比色可视化，具有理论简单，技术要求低。比色法，其利用的颜色变化，所产生的颗粒间等离子体耦合过程中的AuNP的聚集或AuNP聚集体的再分散，已被应用于检测各种各样的目标，包括核酸，蛋白质，小分子，和金属离子。[6]美国
The development of direct, selective, and sensitive assay methods for the detection of physiologically important species involved in the brain function has drawn much attention, because the analytical methods are still the bottleneck for progress in understanding the role of neurotransmitters on neural activity, animal behavior, and so on.[1] Dopamine (DA) is one of the most important neurotransmitters, because it plays a central role in brain functions such as reward-related behavior, movement, and mood.[2] Alternations in neuronal release and uptake of DA have been implicated in psychiatric disorders, addiction, and Parkinson s disease.[3] In the past 30 years, a large number of elegant methods, including electrochemical strategies, spectroscopic approaches, chromatography, and so on, have been employed for DA detection.[1c, 3b, 4, 5] However, the complexity of cerebral systems presents a great challenge to these existing methods to provide theoretically and technically simple DA detection in cerebral systems.Herein, we first report a direct, selective, and sensitive strategy for the colorimetric visualization of cerebral DA at nanomolar levels using gold nanoparticles (AuNPs), with theoretical simplicity and low technical demands. Colorimetric methods, which take advantage of the color change that arises from the interparticle plasmon coupling during the aggregation of AuNPs or the redispersion of AuNP aggregates, have been applied for the detection of a large variety of targets, including nucleic acids, proteins, small molecules, and metal ions.[6]