Cellulose and Collagen Derived Micro-Nano Structured Scaffolds for Bone Tissue Engineering
Cellulose and Collagen Derived Micro-Nano Structured Scaffolds for Bone Tissue Engineering
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DOI：
10.1166/jbn.2013.1574
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发表时间：
2013-04-01
影响因子：
2.9
通讯作者：
Kumbar, Sangamesh G.
中科院分区：
文献类型：
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作者：
Aravamudhan, Aja;Ramos, Daisy M.;Kumbar, Sangamesh G.
基于支架的骨组织工程在修复骨缺损方面取得了很大进展。天然和合成来源的材料已用于支架制造。衍生自天然聚合物的支架由于其与天然细胞外基质（ECM）组分的相似性而提供与哺乳动物组织更大的生物活性和生物相容性以有利于组织愈合。通常，制造用于BTE应用的天然聚合物基支架而不损害其生物活性，同时保持足够的机械性能是一个挑战。在这项工作中，我们报告的制造和表征纤维素和胶原蛋白为基础的微纳米结构的支架使用人成骨细胞（HOB）的BTE应用。这些多孔微纳米结构支架（平均孔径190 +/- 10 μ m）表现出人类松质骨中等范围的机械性能（压缩模量266.75 +/- 33.22 MPa和强度12.15 +/- 2.23 MPa）。这些支架支持培养的HOB的更大的粘附性和表型维持，如与具有相同孔性质的对照聚酯微纳米结构支架相比，成骨酶碱性磷酸酶和矿物质沉积的更高水平所反映的。这些基于天然聚合物的微纳米结构支架可以作为聚酯基支架的替代品用于BTE应用。
Scaffold based bone tissue engineering (BTE) has made great progress in regenerating lost bone tissue. Materials of natural and synthetic origin have been used for scaffold fabrication. Scaffolds derived from natural polymers offer greater bioactivity and biocompatibility with mammalian tissues to favor tissue healing, due to their similarity to native extracellular matrix (ECM) components. Often it is a challenge to fabricate natural polymer based scaffolds for BTE applications without compromising their bioactivity, while maintaining adequate mechanical properties. In this work, we report the fabrication and characterization of cellulose and collagen based micro-nano structured scaffolds using human osteoblasts (HOB) for BTE applications. These porous micro-nano structured scaffolds (average pore diameter 190 +/- 10 mu m) exhibited mechanical properties in the mid range of human trabecular bone (compressive modulus 266.75 +/- 33.22 MPa and strength 12.15 +/- 2.23 MPa). These scaffolds supported the greater adhesion and phenotype maintenance of cultured HOB as reflected by higher levels of osteogenic enzyme alkaline phosphatase and mineral deposition compared to control polyester micro-nano structured scaffolds of identical pore properties. These natural polymer based micro-nano structured scaffolds may serve as alternatives to polyester based scaffolds for BTE applications.