Long-term in vivo evolution of high-purity Mg screw degradation - Local and systemic effects of Mg degradation products
Long-term in vivo evolution of high-purity Mg screw degradation - Local and systemic effects of Mg degradation products
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DOI：
10.1016/j.actbio.2018.02.023
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发表时间：
2018-04-15
影响因子：
9.7
通讯作者：
Sun, Jiao
中科院分区：
文献类型：
--
作者：
Yu, Yiqiang;Lu, Hua;Sun, Jiao
镁（Mg）基材料是骨科和颅颌面外科可降解材料的研究热点。然而，腐蚀速率控制和生物安全仍然是其临床应用之前需要解决的关键问题。在本研究中，将轧制高纯度镁（HP Mg，99.99 wt%）螺钉植入兔胫骨中长达 52 周，以研究其体内长期降解及其降解产物的局部和全身影响。在植入后的不同时间点（4周、12周、26周和52周）进行了一系列长期监测，采用显微CT检测、组织形态学分析、局部微环境测试以及血清和尿液的生化分析等多种研究。结果表明，HP Mg螺钉在52周内具有均匀的降解形态和缓慢的体内降解速率。它们的降解产物不仅增加了局部pH值，而且动态改变了种植体周围组织中局部Mg2+离子浓度和气腔面积。更重要的是，植入后26周和52周时，骨植入界面的新骨形成和骨植入接触率均有所增加。此外，在26周的监测期间，未发现血清镁和尿镁水平异常升高，也未发现肝肾功能障碍。所有这些长期研究的结果表明，HP Mg螺钉具有缓慢的降解速度、理想的骨修复能力和长期的局部/全身生物安全性，因此可能具有作为骨固定装置的良好应用潜力。 (C) 2018 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Magnesium (Mg) based materials are the focus of research for use as degradable materials in orthopedics and cranio-maxillofacial surgery. However, corrosion rate control and biosecurity are still the key issues that need to be solved prior to their clinical applications. In the present study, as-rolled high-purity magnesium (HP Mg, 99.99 wt%) screws were implanted in rabbit tibiae for up to 52 weeks in order to investigate their long-term in vivo degradation and the local and systemic effects of their degradation products. A series of long-term monitoring were performed at various time points (4w, 12w, 26w and 52w) after implantation using numerous investigations such as micro-CT assay, histomorphometric analysis, local micro-environment testing and biochemical analysis of serum and urine. It was revealed that HP Mg screws had a uniform degradation morphology and a slow degradation rate in vivo during the period of 52 weeks. Their degradation products not only increased the local pH values but also changed the local Mg2+ ions concentration and gas cavity area in the peri-implant tissues in a dynamic manner. More importantly, both the new bone formation and bone-implant contact rate were increased at bone implant interfaces at 26 weeks and 52 weeks post-implantation. Furthermore, neither abnormal elevation of serum magnesium and urine magnesium level, nor liver and kidney dysfunction were detected during the monitoring period of 26 weeks. All these results of long-term investigation suggest that HP Mg screws possess a slow degradation rate, desirable bone repair capacity and long-term local/systemic biosafety, and consequently may have good potential for application as bone fixation devices. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.