RNA-protein interactions and secondary structures of cowpea chlorotic mottle virus for in vitro assembly.
RNA-protein interactions and secondary structures of cowpea chlorotic mottle virus for in vitro assembly.
复制标题
用于体外组装的豇豆褪绿斑驳病毒的RNA-蛋白质相互作用和二级结构。
DOI：
10.1021/bi00314a008
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
ThomasJr,GJ
中科院分区：
文献类型：
--
作者：
Verduin,BJ;Prescott,B;ThomasJr,GJ
B。J. M. Verduin，B. Prescott和G.小托马斯 *摘要：豇豆褪绿斑驳病毒（CCMV）在自然状态（pH 5.0）和部分溶胀状态（pH 7.5）下的激光拉曼光谱揭示了在自然病毒粒子的双核苷酸化RNA分子中存在小百分比的质子化腺嘌呤（< 15%）和胞嘧啶（< 7%）碱基。质子化碱用pH诱导的病毒溶胀滴定。在相同的pH范围内不能检测到病毒体亚基的天冬氨酸和谷氨酸侧链的推定COOH基团的滴定，这表明羧基阴离子（CO2-）和质子化碱在pH 5下均可通过静电相互作用稳定核糖核蛋白颗粒。高度（95%）有序的二级结构的重组RNA可能经历一个小的额外增加（< 3%）无序结构从病毒体释放，最多表明一个边际结构扭曲的影响
B. J. M. Verduin, B. Prescott, and G. J. Thomas, Jr.* abstract: Laser Raman spectroscopy of the cowpea chlorotic mottle virus (CCMV) in native (pH 5.0) and partially swollen (pH 7.5) states reveals the presence of small percentages of protonated adenine (< 15%) and cytosine (< 7%) bases in the encapsidated RNA molecule of the native virion. The pro-tonated bases are titrated with pH-inducedswelling of the virus. Titration of putative COOH groups of aspartic and glutamic side chains of the virion subunit cannot be detected over the same pH range, which suggests that carboxyl anions (C02~) and protonated bases are both available at pH 5 to stabilize the ribonucleoprotein particles by electrostatic interactions. The highly (95%) ordered secondary structure of encapsidated RNA may undergo a small additional increase (< 3%) inordered structure with release from the virion, suggesting at most a marginal structure-distorting influence