CONCLUSIVE EVIDENCE THAT VERY LONG-CHAIN FATTY-ACIDS ARE OXIDIZED EXCLUSIVELY IN PEROXISOMES IN HUMAN SKIN FIBROBLASTS
CONCLUSIVE EVIDENCE THAT VERY LONG-CHAIN FATTY-ACIDS ARE OXIDIZED EXCLUSIVELY IN PEROXISOMES IN HUMAN SKIN FIBROBLASTS
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DOI：
10.1016/0006-291x(91)90967-c
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发表时间：
1991-08-15
影响因子：
3.1
通讯作者：
WANDERS, RJA
中科院分区：
文献类型：
--
作者：
JAKOBS, BS;WANDERS, RJA
我们研究了过氧化物酶体和线粒体在完整的人皮肤成纤维细胞中棕榈酸酯（C16:0）和棕榈酸酯（C26:0）的β-氧化中的作用。鱼藤酮加抗霉素和氰化物分别抑制了这两种脂肪酸的氧化，尽管程度不同。用2-[5-（4-氯苯基）戊基]-氧烷-2-羧酸酯（POCA）特异性阻断肉碱棕榈酰基转移酶I时，发现棕榈酸β-氧化几乎完全被抑制，而棕榈酸β-氧化不受影响。由于肉毒碱棕榈酰基转移酶对线粒体中脂肪酸的氧化至关重要，这一结果提供了确凿的证据，证明长链脂肪酸的氧化是在过氧化物酶体中而不是在线粒体中开始的。
We have investigated the contribution of peroxisomes and mitochondria to the β-oxidation of palmitate (C16:0) and cerotate (C26:0) in intact human skin fibroblasts. The oxidation of both fatty acids was found to be inhibited by rotenone plus antimycin and cyanide, respectively, although to a different extent. When 2-[5-(4-chlorophenyl)pentyl]-oxirane-2-carboxylate (POCA) was used to specifically block carnitine palmitoyltransferase I, it was found that palmitate β-oxidation was inhibited almost completely whereas cerotate β-oxidation was not affected. Since carnitine palmitoyltransferase is essential for the oxidation of fatty acids in mitochondria this result provides conclusive evidence that oxidation of very-long-chain fatty acids is initiated in peroxisomes and not in mitochondria.