Cultivation of Escherichia coli to high cell densities in a dialysis reactor
Cultivation of Escherichia coli to high cell densities in a dialysis reactor
复制标题
在透析反应器中培养大肠杆菌至高细胞密度
DOI：
10.1007/bf00166847
复制
发表时间：
1993
影响因子：
5
通讯作者：
J. Ogbonna
中科院分区：
文献类型：
--
作者：
H. Märkl;C. Zenneck;A. Dubach;J. Ogbonna
研究了在甘油基矿物培养基上大肠杆菌的高细胞密度培养。培养在由两个室组成的透析反应器中进行。内室由膜形成并与外室隔开。新鲜培养基与外室中的培养基不断交换，使得甘油和培养基的其他成分通过膜供应至内室。抑制物质从内室扩散到外室，随后随外室的流出物一起被去除。最初，使用数学模型来描述该过程。通过初步实验和计算确定了最佳培养参数，例如两个室中的初始甘油浓度、所需的跨膜转运速率、进料/透析介质中的甘油浓度以及开始介质交换的时间。实际栽培结果与模型预测非常吻合。获得了 174 g 干重/1 的非常高的细胞浓度。该细胞浓度在培养液中大肠杆菌的最大理论浓度 (160–200 g/l) 范围内。
High cell density cultivation of Escherichia coli on a glycerol-based mineral medium was studied. The cultivation was done in a dialysis reactor composed of two chambers. The inner chamber is formed and separated from an outer chamber by a membrane. Fresh medium was continuously exchanged with medium in the outer chamber so that both glycerol and other components of the medium were supplied to the inner chamber through the membrane. Inhibitory substances diffused from the inner to the outer chamber and were subsequently removed with effluent from the outer chamber. Initially, mathematical models were used to describe the process. The optimal cultivation parameters, such as the initial glycerol concentrations in the two chambers, the desired transport rate across the membrane, glycerol concentration in the feed/dialysing medium, and the time to start the medium exchange, were determined from preliminary experiments and calculations. The actual cultivation results agreed very well with the model predictions. A very high cell concentration of 174 g dry weight/1 was obtained. This cell concentration is within the range of the maximum theoretical concentration of E. coli in culture broth (160–200 g/l).