Determination of the optimal human cell lines for development of human hybridomas.
Determination of the optimal human cell lines for development of human hybridomas.
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确定用于开发人类杂交瘤的最佳人类细胞系。
DOI：
10.4049/jimmunol.131.3.1201
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发表时间：
1983
影响因子：
4.4
通讯作者：
K. Foon
中科院分区：
文献类型：
--
作者：
P. Abrams;J. Knost;G. Clarke;S. Wilburn;R. Oldham;K. Foon
将六种人类细胞系相互比较并与鼠骨髓瘤 NS-1 比较它们对 HAT 培养基的敏感性以及它们与人类淋巴细胞形成杂交体、分泌单克隆免疫球蛋白、克隆以及在融合后一段时间内维持可检测水平的单克隆免疫球蛋白分泌的能力。杂交体的融合效率从 0% 到 50% 不等，免疫球蛋白分泌的发生率在 1% 到 78% 之间。克隆杂交体的免疫球蛋白分泌在人-人杂交体中为0.8至1.6微克/ml/10(6)细胞，而在人-小鼠杂交体中为2.4微克/ml/10(6)细胞。在测试的细胞系中，UC729-6 和 HF2 似乎最适合进行人-人杂交瘤的进一步研究。此外，虽然只有一小部分的杂交体与HMy2产生，但分泌免疫球蛋白的比例非常高，因此该品系也值得进一步研究以提高杂交体形成的效率。讨论了特异性单克隆抗体生产以及抗独特型抗体治疗白血病和淋巴瘤的意义。
Six human cell lines were compared with each other and with murine myeloma NS-1 as to their sensitivity to HAT medium and their ability to form hybrids with human lymphocytes, secret monoclonal immunoglobulin, clone, and maintain detectable levels of monoclonal immunoglobulin secretion for a period of time after fusion. Fusion efficiencies varied from 0 to 50%, and the incidence of immunoglobulin secretion ranged between 1 and 78% of the hybrids. Immunoglobulin secretion of cloned hybrids varied from 0.8 to 1.6 micrograms/ml/10(6) cells among the human-human hybrids and was 2.4 micrograms/ml/10(6) cells by the human-mouse hybrid. Among the lines tested, UC729-6 and HF2 appeared optimal for pursuing further studies with human-human hybridomas. In addition, although only a small percentage of hybrids were produced with HMy2, a very high percentage secreted immunoglobulin, so that this line also warrants further investigation to improve the efficiency of hybrid formation. Implications for specific monoclonal antibody production and for therapy of leukemias and lymphomas by anti-idiotype antibodies are discussed.