Polo boxes and Cut23 (Apc8) mediate an interaction between polo kinase and the anaphase-promoting complex for fission yeast mitosis.
Polo boxes and Cut23 (Apc8) mediate an interaction between polo kinase and the anaphase-promoting complex for fission yeast mitosis.
复制标题
Polo 盒和 Cut23 (Apc8) 介导 polo 激酶和分裂酵母有丝分裂后期促进复合物之间的相互作用。
DOI：
10.1083/jcb.200106150
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发表时间：
2002-01-07
影响因子：
7.8
通讯作者：
Ohkura, Hiroyuki
中科院分区：
文献类型：
--
作者：
May, Karen M;Reynolds, Nicola;Cullen, C Fiona;Yanagida, Mitsuhiro;Ohkura, Hiroyuki
分裂酵母plo1 +基因编码一种polo样激酶，这是一个保守的激酶家族的成员，在细胞周期中发挥多种作用。我们发现Plo1激酶通过Plo1的非催化结构域和亚基Cut23的四肽重复结构域与后期促进复合物(APC)/环体物理相互作用。一个新的cut23突变，特别破坏了与Plo1的相互作用，导致中期阻滞。Plo1激酶的高表达可以挽救这种阻滞。我们认为这种物理相互作用对有丝分裂的进展至关重要，通过靶向polo激酶活性到APC。
The fission yeast plo1 + gene encodes a polo-like kinase, a member of a conserved family of kinases which play multiple roles during the cell cycle. We show that Plo1 kinase physically interacts with the anaphase-promoting complex (APC)/cyclosome through the noncatalytic domain of Plo1 and the tetratricopeptide repeat domain of the subunit, Cut23. A new cut23 mutation, which specifically disrupts the interaction with Plo1, results in a metaphase arrest. This arrest can be rescued by high expression of Plo1 kinase. We suggest that this physical interaction is crucial for mitotic progression by targeting polo kinase activity toward the APC.