Failure to document fetal cells in maternal circulation using the Selypes-Lorencz "air-culture" cytogenetic technique.
Failure to document fetal cells in maternal circulation using the Selypes-Lorencz "air-culture" cytogenetic technique.
复制标题
使用 Selypes-Lorencz“空气培养”细胞遗传学技术未能记录母体循环中的胎儿细胞。
DOI：
10.1007/bf00276341
复制
发表时间：
1990
期刊：
影响因子：
5.3
通讯作者：
Elias,S
中科院分区：
文献类型：
--
作者：
Youssef,M;Shulman,LP;Tharapel,AT;Simpson,JL;Elias,S
分离和分析在母体循环中循环的胎儿细胞将允许相对非侵入性的方法（即，静脉穿刺）进行产前细胞遗传学诊断。有几个研究小组声称有证据表明母体循环中存在胎儿细胞，在一份报告中，Selypes和Lorencz（1988年）报告说，在来自孕妇的植物血凝素刺激的血培养物中存在相对大量的胎儿有丝分裂细胞。我们采用Selypes和Lorencz的实验室方法评估了29例患者（妊娠9.7至19.5周）的母血，其中胎儿补体与母亲不同（例如，46，XY，非整倍性）。不幸的是，我们无法确认Selypes和Lorencz的结果。
Isolating and analyzing fetal cells circulating in the maternal circulation would allow a relatively noninvasive method (i.e., venipuncture) for prenatal cytogenetic diagnosis. Several groups have claimed evidence for the presence of fetal cells in maternal circulation, and in one communication, Selypes and Lorencz (1988) reported the presence of relatively large numbers of fetal mitotic cells in phytohemagglutinin-stimulated blood cultures derived from pregnant women. We employed the laboratory method of Selypes and Lorencz in evaluating maternal blood from 29 patients (9.7 to 19.5 weeks gestation) in which the fetal complement differed from that of the mother (e.g., 46,XY, aneuploidy). Unfortunately, we were unable to confirm the results of Selypes and Lorencz.