Enhancers and trans-acting E2 transcriptional factors of papillomaviruses.
Enhancers and trans-acting E2 transcriptional factors of papillomaviruses.
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乳头瘤病毒的增强子和反式作用 E2 转录因子。
DOI：
10.1128/jvi.61.8.2599-2606.1987
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发表时间：
1987
影响因子：
5.4
通讯作者：
Chow,LT
中科院分区：
文献类型：
--
作者：
Hirochika,H;Broker,TR;Chow,LT
将人乳头瘤病毒（HPV）1型、6 b型、7型、11型、16型和18型、牛乳头瘤病毒1型和棉尾兔乳头瘤病毒的上游调控区克隆到转录增强子测定质粒中，该质粒携带缺乏自身增强子的猿猴病毒40早期启动子和编码氯霉素乙酰转移酶的细菌基因（EC 2.3.1.28）（CAT）。只有当重组体与表达HPV 1型和11型或牛乳头瘤病毒1型的完整E2开放阅读框的质粒共转染时，才能在所有测试的上游调控区中检测到CAT基因表达所反映的增强子活性。每个E2蛋白刺激来自相同病毒的增强子，并且在某种程度上，也刺激来自异源病毒的增强子。因此，增强子和E2蛋白在乳头瘤病毒中是功能保守的。在异源E2-增强子相互作用中反式激活的程度存在一些非互惠性。引物延伸分析表明，E2蛋白增加了CAT基因mRNA的丰度。HPV 11型增强子序列的串联倍增显著增加了其对E2刺激的反应;这可能与乳头瘤病毒的致病性有关。
The upstream regulatory regions of human papillomavirus (HPV) types 1, 6b, 7, 11, 16, and 18, bovine papillomavirus type 1, and cottontail rabbit papillomavirus were cloned into transcriptional enhancer assay plasmids which carry the simian virus 40 early promoter lacking its own enhancer and the bacterial gene encoding chloramphenicol acetyltransferase (EC 2.3.1.28) (CAT). Enhancer activity, reflected by CAT gene expression, was detected in all of the upstream regulatory regions tested only when the recombinants were cotransfected with plasmids which express an intact E2 open reading frame of HPV types 1 and 11 or bovine papillomavirus type 1. Each E2 protein stimulated the enhancer from the same virus and, to somewhat lesser degrees, also those from the heterologous viruses. Hence, the enhancer and the E2 protein are functionally conserved among papillomaviruses. There was some nonreciprocity in the extent of trans-activation in heterologous E2-enhancer interactions. Primer extension analyses demonstrated that the E2 proteins increased the abundance of CAT gene mRNA. Tandem multiplication of the HPV type 11 enhancer sequence dramatically increased its response to E2 stimulation; this is possibly relevant to the pathogenicity of papillomaviruses.