Direct injection of immature dendritic cells into irradiated tumor induces efficient antitumor immunity
Direct injection of immature dendritic cells into irradiated tumor induces efficient antitumor immunity
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DOI：
10.1002/ijc.20036
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发表时间：
2004-05-01
影响因子：
6.4
通讯作者：
Kang, CD
中科院分区：
文献类型：
--
作者：
Kim, KW;Kim, SH;Kang, CD
虽然有多种方法可以将肿瘤抗原负载到树突状细胞上，但通常需要体外制备肿瘤抗原和操纵树突状细胞。因此，为了开发一种简单的抗肿瘤免疫方法，我们研究了电离IR诱导的DC直接注射到肿瘤内是否能诱导有效的抗肿瘤免疫。电离辐射15Gy6小时后诱导肿瘤细胞凋亡作用最强。注射树突状细胞I.T.TO-IR瘤诱导的脾细胞对肿瘤细胞的杀伤作用强于I.T.注射树突状细胞或肿瘤电离IR，均有较弱的细胞毒作用。在一项动物研究中，IT。将树突状细胞注射到IR肿瘤中，可诱导针对远处肿瘤的治疗性抗肿瘤免疫。此外，当加入肿瘤坏死因子-α或内毒素作为危险/成熟信号时，DC悬液在I.T.与I.T.组相比，注射组的抗肿瘤免疫力显著增强。胰岛素抵抗瘤内注射树突状细胞。结果提示，电离IR诱导的DC瘤内注射可能是一种简单有效的肿瘤免疫方法。(C)2004年Wiley-Liss公司
Although there are several ways to load tumor antigens to DCs, in vitro preparation of tumor antigens and manipulation of DCs are usually required. Therefore, to develop a simple antitumor immunization method, we examined if direct injection of DCs into tumor apoptosed by ionizing IR could induce efficient antitumor immunity. Ionizing IR with 15 Gy induced apoptosis in tumor maximally after 6 hr. Injection of DCs i.t. into IR tumor induced strong cytotoxicity of splenocytes against tumor cells compared to i.t. injection of DCs or ionizing IR of tumor, both of which induced weak cytotoxicity. In an animal study, i.t. injection of DCs into IR tumor induced therapeutic antitumor immunity against a tumor established at a distant site. Moreover, when TNF-alpha or LPS was added as a danger/maturation signal to DC suspension before i.t. injection, antitumor immunity was significantly potentiated compared to a group treated with i.t. injection of DCs into IR tumor. Our results suggest that injection of DCs into tumor apoptosed by ionizing IR might be a simple and efficient method of immunization against tumor. (C) 2004 Wiley-Liss, Inc.