Expression of the mitochondrial genome in xenopus laevis: a map of transcripts
Expression of the mitochondrial genome in xenopus laevis: a map of transcripts
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非洲爪蟾线粒体基因组的表达：转录本图谱
DOI：
10.1016/0092-8674(79)90067-9
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发表时间：
1979
期刊：
影响因子：
64.5
通讯作者：
I. Dawid
中科院分区：
文献类型：
--
作者：
E. Rastl;I. Dawid
对滑鼠线粒体中 12 个最丰富的转录本进行了表征，并通过电子显微镜中的 R 环作图和 RNA 凝胶转移杂交将其编码区定位在线粒体 DNA (mtDNA) 上。除一处例外外，转录本位于不重叠的位置，它们约占线粒体 DNA 编码能力的 80%。 12 个 RNA 分子中有 10 个含有聚 (A)；两个缺乏poly(A)的转录物是rRNA。单链特异性核酸酶分析清楚地表明，7 个 RNA 的编码区不存在干扰序列。对于两种 RNA，强烈建议使用不间断的编码序列，而一种 RNA 无法分析。对八个低丰度和高分子量的转录本进行了表征，并大致绘制了它们的编码区。它们与丰富的线粒体 RNA 的编码区重叠，并且可能是这些 RNA 的前体。大多数表征的 RNA 分子均由 mtDNA 重链转录而来。没有发现丰富的离散轻链转录本。
The twelve most abundant transcripts in X. laevis mitochondria were characterized, and their coding regions were mapped on the mitochondrial DNA (mtDNA) by R loop mapping in the electron microscope and by RNA gel transfer hybridization. The transcripts map in nonoverlapping positions with one exception, and they account for about 80% of the coding capacity of mtDNA. Ten of the twelve RNA molecules contain poly(A); the two poly(A)-lacking transcripts are the rRNAs. Analysis with single-strand-specific nucleases clearly demonstrated the absence of intervening sequences from the coding regions for seven RNAs. For two RNAs, uninterrupted coding sequences are strongly suggested and one RNA could not be analyzed. Eight transcripts of low abundance and high molecular weight were characterized, and their coding regions were mapped approximately. They overlap the coding regions of the abundant mitochondrial RNAs and could be precursors of these RNAs. Most of the RNA molecules characterized were shown to be transcribed from the heavy strand of mtDNA. No abundant discrete light-strand transcript was found.