A genome scan with AFLP™ markers to detect fearfulness-related QTLs in Japanese quail
A genome scan with AFLP™ markers to detect fearfulness-related QTLs in Japanese quail
复制标题
DOI：
10.1111/j.1365-2052.2005.01336.x
复制
发表时间：
2005-10-01
期刊：
影响因子：
2.4
通讯作者：
Vignal, A
中科院分区：
文献类型：
--
作者：
Beaumont, C;Roussot, O;Vignal, A
通过数量性状基因座（QTL）研究来确定日本鹌鹑（Coturnix japonica）恐惧控制相关的基因组区域。一个F2杂交的两个鹌鹑线之间分歧选择超过29代的持续时间的紧张性不动（DTI），紧张样状态的反应性降低的压力刺激。共测量了1065只动物的DTI对数（LOGTI）、诱导不动反应所需的诱导次数（NI）、旷场行为，包括自发活动（MOVE）、第一次运动前的潜伏期（LAT）、跳跃次数（JUMP）、沮丧次数（DEJ）和喊叫次数（SHOUT），竞争应激后皮质酮水平（LOGCORT）和2周龄体重（BW 2）。总共有310只动物被包括在使用248个AFLP标记的选择性基因分型的基因组扫描中。共检测到21个QTL，其中包括21个在全基因组范围内具有显著性或暗示性的QTL。在连锁群1（GL1）上检测到两个极显著的QTL，一个为LOGTI，一个为NI。在LOGTI的QTL附近，还鉴定了一个几乎显著的SHOUT QTL和一个LAT的暗示性QTL。在GL3上，JUMP和DEJ检测到全基因组显著性QTL，LOGTI、MOVE、SHOUT和LAT检测到提示性QTL。BW2在GL2上有一个显著的QTL，在GL1上有一个接近显著的QTL。这些结果可能有助于理解鹌鹑和相关物种的恐惧，前提是恐惧具有相同的遗传基础。
A quantitative trait loci (QTL) study was undertaken to identify genome regions involved in the control of fearfulness in Japanese quail (Coturnix japonica). An F2 cross was made between two quail lines divergently selected over 29 generations on duration of tonic immobility (DTI), a catatonic-like state of reduced responsiveness to a stressful stimulation. A total of 1065 animals were measured for the logarithm of DTI (LOGTI), the number of inductions (NI) necessary to induce the immobility reaction, open-field behaviour including locomotor activity (MOVE), latency before first movement (LAT), number of jumps (JUMP), dejections (DEJ) and shouts (SHOUT), corticosterone level after a contention stress (LOGCORT) and body weight at 2 weeks of age (BW2). A total of 310 animals were included in a genome scan using selective genotyping with 248 AFLP markers. A total of 21 suggestive or genome-wide significant QTL were observed. Two highly significant QTL were identified on linkage group 1 (GL1), one for LOGTI and one for NI. In the vicinity of the QTL for LOGTI, a nearly significant QTL for SHOUT and a suggestive QTL for LAT were also identified. On GL3, genome-wide significant QTL were observed for JUMP and DEJ as well as suggestive QTL for LOGTI, MOVE, SHOUT and LAT. A significant QTL for BW2 was observed on GL2 and a nearly significant one on GL1. These results may be useful in the understanding of fearfulness in quail and related species provided that fearfulness has the same genetic basis.