Development of a bioassay system for monitoring susceptibility in Thrips tabaci.
Development of a bioassay system for monitoring susceptibility in Thrips tabaci.
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DOI：
10.1002/ps.680
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发表时间：
2003-05
影响因子：
4.1
通讯作者：
A. Rueda;A. Shelton
中科院分区：
文献类型：
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作者：
A. Rueda;A. Shelton
描述了一种从洋葱叶上收集烟蓟马成虫和幼虫的系统，以评估对杀虫剂的敏感性。蓟马杀虫剂生物测定系统（TIBS）允许在收集蓟马之前处理小瓶并将其储存3周。根据田间的种群密度，一个人需要收集3-6小时的昆虫用于测试。在24小时后读取分析。该系统被用于1997年和1998年在洪都拉斯和尼加拉瓜的商业洋葱领域，和TIBS是足够敏感的检测差异的杀虫剂测试，蓟马的生命阶段和洋葱生长季节内的不同世代。收集到的数据表明，蓟马杀虫剂抗药性没有严重问题，但尼加拉瓜的氯氰菊酯可能例外，其成虫的抗药性比率（RR）值为26.9。最大的RR值观察到在生长季节结束时，这可能是由杀虫剂喷洒的季节选择。成虫和幼虫的死亡率遵循相同的一般模式，但幼虫和成虫之间的比例对于每个化学品组不同。
A system is described for collecting adult and larva of Thrips tabaci from onion foliage into insecticide-treated vials to evaluate susceptibility to insecticides. The thrips insecticide bioassay system (TIBS) allows one to treat vials and store them for 3 weeks before thrips are collected. Depending on the population density in the field, collection of the insects for the test required from 3-6 h for one person. Assays are read after 24h. This system was used in 1997 and 1998 in commercial onion fields in Honduras and Nicaragua, and TIBS was sensitive enough to detect differences to the insecticides tested, to thrips life stages and to different generations within an onion-growing season. Data collected suggest that there were not serious problems with thrips insecticide resistance, with the possible exception of cypermethrin in Nicaragua which had a resistance ratio (RR) value of 26.9 for adult thrips. The largest RR values were observed at the end of the growing season, and this may be caused by the season-long selection by insecticide sprays. The mortality of adults and larvae followed the same general pattern, but the ratio between larvae and adults differed for each chemical group.