Preferential DNA Repair in Human Cells
Preferential DNA Repair in Human Cells
复制标题
人体细胞中的优先 DNA 修复
DOI：
10.1038/247035a0
复制
发表时间：
1974
期刊：
影响因子：
64.8
通讯作者：
Ronald W. Hart
中科院分区：
文献类型：
--
作者：
R. J. Wilkins;R. J. Wilkins;Ronald W. Hart
切除修复在人类细胞从紫外线辐射中恢复方面起着至关重要的作用，但它并不能消除DNA上的所有损伤，即使它们与环丁烷嘧啶二聚体一样有效。事实上，只有大约50%到75%的由低通量的紫外光产生的二聚体被切除，其余的在DNA中至少保留24到48个H2-4。我们已经研究了为什么一些而不是所有的二聚体被切除。我们的结论是，二聚体，可能还有其他紫外线光产物，持续存在于DNA片断中，这些DNA片断无法通过蛋白质的“面具”进行切除修复。
EXCISION repair plays a vital role in the recovery of human cells from ultraviolet irradiation1, but it does not remove all lesions from DNA, even when they are as potent as cyclobutane pyrimidine dimers. In fact, only about 50% to 75% of the dimers produced by low fluences of ultraviolet light are excised and the remainder persist in the DNA for at least 24 to 48 h2–4. We have investigated why some but not all dimers are excised. Our conclusion is that dimers, and possibly other ultraviolet photoproducts, persist in tracts of DNA which are rendered refractory to excision repair by a ‘mask’ of protein.