Breeding for Bio-ethanol Production in Lolium perenne L.: Association of Allelic Variation with High Water-Soluble Carbohydrate Content.
Breeding for Bio-ethanol Production in Lolium perenne L.: Association of Allelic Variation with High Water-Soluble Carbohydrate Content.
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DOI：
10.1007/s12155-011-9156-0
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发表时间：
2012
影响因子：
3.6
通讯作者：
Donnison IS
中科院分区：
文献类型：
--
作者：
Farrar K;Bryant DN;Turner L;Gallagher JA;Thomas A;Farrell M;Humphreys MO;Donnison IS
增加多年生作物的可提取糖产量是生产生物乙醇等可再生燃料的一种战略。多年生黑麦草(多年生黑麦草)含有大量(30%干物质)水溶性糖，以聚合果糖的形式存在，很容易提取、分解并发酵成生物乙醇。从4个不同水溶性碳水化合物(WSC)含量的亲本中产生的多年生黑麦草群体，在早春WSC含量的基础上进行了多轮选择和重组，以产生高WSC和低WSC群体。通过随机选择相同数量的植株来产生对照群体。对选择前后的6个候选基因座的等位基因进行分析，并与WSC含量进行相关分析。多年生黑麦草可溶性酸性转化酶1：4等位基因频率在三个群体之间存在显著差异，其中一个单倍型与高WSC C2S+群体显著相关(经过三轮选择和两轮重组)。此外，WSC含量还与生物量积累有关。因此，除了WSC产量增加2.84倍外，C2S+群体的单株生物量也增加了1.48倍，导致每株WSC产量比对照群体高3.9倍。本文的在线版本(doi：10.1007/s12155.0119156-0)包含补充材料，授权用户可以使用。
Increasing the extractable sugar yield from perennial crops is one strategy to generate renewable fuels such as bio-ethanol. Lolium perenne L. (perennial ryegrass) can contain significant (>30% dry matter) water-soluble sugars in the form of polymeric fructan which is readily extracted, broken down and fermented to bio-ethanol. A population of L. perenne generated from four parents which differed in water-soluble carbohydrate (WSC) content was subjected to multiple rounds of selection and recombination on the basis of early spring WSC content to produce a high WSC, and a low WSC population. A control population was generated by selecting the same number of plants at random. The alleles present at six candidate gene loci were analysed before and after selection and correlated to WSC content. Significant differences in the allele frequency of L. perenne soluble-acid invertase1:4 were observed between the three populations with one haplotype significantly associated with the high WSC C2S+ population (after three rounds of selection and two rounds of recombination). Moreover, WSC content was also associated with biomass accumulation. Thus, in addition to a 2.84-fold increase in WSC yield, the C2S+ population also had 1.48-fold more biomass per plant, resulting in 3.9-fold higher WSC yield per plant than the control population. The online version of this article (doi:10.1007/s12155-011-9156-0) contains supplementary material, which is available to authorized users.