Comparative plastid genomics of Mazaceae: focusing on a new recognized genus, Puchiumazus
Comparative plastid genomics of Mazaceae: focusing on a new recognized genus, Puchiumazus
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DOI：
10.1007/s00425-021-03753-7
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发表时间：
2021-11-01
期刊：
影响因子：
4.3
通讯作者：
Yu，Jie
中科院分区：
文献类型：
--
作者：
Zeng，Siyuan;Li，Jingling;Yu，Jie
本研究共收集了6个马扎科植物的质体体，其中包括新发现的Puchiumazus属。比较质体体基因组分析为研究马扎科植物提供了新的视角。摘要长期以来，马蹄莲属(Mazus Lanceifolius)的系统发育分类一直是不确定的。2021年，李伯利、张东国、向春林等人发表了《M。Lanceifolius为一新种--Puchiumazus lanceifolius，隶属于Puchiumazus一新属。然而，有关马豆科植物的质体组信息却很少。随着新属Puchiumazus的发表，现在有必要研究马扎科的质组特征，以全面了解这个年轻的家族。马扎科植物的质体体均具有典型的四分位数结构。这些质体体的大小从152,388到154,252个碱基不等，每个质体体包含112个独特的基因，包括78个蛋白质编码基因，4个rRNA基因和30个tRNA基因。比较分析表明，这些质体体序列高度保守。此外，我们还确定了四个相对高变区(trnQ-uuc-psbK、trns-gcu-trns-cga、trnT-ugu-trnL-uaa和ycf1)，它们可以作为潜在的DNA条形码用于该分支的鉴定。基于Lamiales PlacedM.14属25个标本质体全序列的系统发育关系。在马蹄草科的基本支系中，有100%的自举支持。总而言之，他们。Lanceifolius结果表明，应在整个质体组水平上建立一个新的单型属(Puchiumazus)。本研究为探索马扎科植物的系统发育提供了叶绿体基因组资源。
Main conclusionSix Mazaceae plastomes were assembled in this study, including the newly recognized genus,Puchiumazus. Comparative plastid genomic analysis provided new insights into Mazaceae.AbstractThe phylogenetic categorization ofMazus lanceifolius(Mazaceae) has long been uncertain. In 2021, the scholars Bo Li, D. G. Zhang, and C. L. Xiang republishedM. lanceifoliusas a new speciesPuchiumazus lanceifolius,within a new genusPuchiumazus. However, there is little plastome information on Mazaceae. Following the publishing of the new genusPuchiumazus, it is now necessary to study the Mazaceae plastome features to comprehensively understand this young family. The Mazaceae plastomes all have a typical quartile structure. The plastomes have a size ranging from 152,388 to 154,252 bp, and each plastome contains 112 unique genes, including 78 protein-coding genes, 4 rRNA genes, and 30 tRNA genes. A comparative analysis showed that these plastome sequences are highly conserved. Furthermore, we identified four relatively hypervariable regions (trnQ-UUC-psbK,trnS-GCU-trnS-CGA,trnT-UGU-trnL-UAA andycf1) that can be used as potential DNA barcodes for the identification of this clade. Phylogenetic relationships based on the whole plastome sequences of 25 samples of 14 genera of Lamiales placedM. lanceifoliusin the basal clade of the family Mazaceae, with 100% bootstrap support. In summary, theM. lanceifoliusresults indicate that a new monotype genus (Puchiumazus) should be established at the whole-plastome level. This study provides plastid genomic resources for exploring the phylogeny of Mazaceae.