Conditions that support long-term production of osteoclast progenitors in vitro.
Conditions that support long-term production of osteoclast progenitors in vitro.
复制标题
支持破骨细胞祖细胞在体外长期产生的条件。
DOI：
10.1002/stem.150340
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Lottsfeldt,JL
中科院分区：
文献类型：
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作者：
Lee,MY;Fevold,KL;Muguruma,Y;Lottsfeldt,JL
为了更好地了解破骨细胞前体细胞从造血干细胞发育的机制，我们研究了支持破骨细胞祖细胞，破骨细胞集落形成单位（CFU-O）的生产的条件，从骨髓（Dexter's）和淋巴（WhitlockandWitte's）条件下建立的长期骨髓培养。每周从骨髓或淋巴长期培养物中收获的非粘附细胞在存在鼠破骨细胞集落刺激因子的琼脂中测定CFU-O衍生集落形成。骨髓系统支持CFU-O产生数周，但该系统也产生许多其他类型的骨髓集落和细胞，并且CFU-O衍生集落的定量是困难的。淋巴长期培养系统也产生CFU-O;然而，淋巴系统中CFU-O的产生似乎比骨髓系统中更具选择性，但是短暂的。有趣的是，向这些培养物中加入含有G-CSF的培养基大大提高了CFU-O的产生（>200%）。这种增强的CFU-O产生使用在淋巴条件下在确定的骨髓基质细胞系上建立并补充有重组鼠G-CSF的骨髓培养物来证实。因此，G-CSF促进在淋巴长期培养条件下从造血干细胞发育成克隆性破骨细胞祖细胞。该培养体系可作为体外培养破骨细胞祖细胞的有效模型。
To better understand the mechanisms of osteoclast precursor development from hematopoietic stem cells, we examined the conditions that support the production of osteoclast progenitors, osteoclast colony-forming units (CFU-O), from long-term bone marrow cultures established under myeloid (Dexter's) and lymphoid (WhitlockandWitte's) conditions. Nonadherent cells harvested weekly from myeloid or lymphoid long-term cultures were assayed for CFU-O-derived colony formation in agar in the presence of a murine osteoclast colony-stimulating factor. The myeloid system supported CFU-O production for weeks, but the system produced many other types of myeloid colonies and cells as well, and quantification of CFU-O-derived colonies was difficult. The lymphoid long-term culture system also produced CFU-O; however, CFU-O production in the lymphoid system appeared more selective than in the myeloid system, but was transient. Interestingly, the addition of medium containing G-CSF to these cultures greatly enhanced (>200%) the CFU-O production. This enhanced CFU-O production was confirmed using bone marrow cultures established on a defined marrow stromal cell line under lymphoid conditions and supplemented with recombinant murine G-CSF. Thus, G-CSF facilitates the development of clonogenic osteoclast progenitors from hematopoietic stem cells in lymphoid long-term culture conditions. This culture system may serve as a useful model for ex vivo generation of osteoclast progenitors.