PRESENCE OF TYPE-III COLLAGEN IN DISK ATTACHMENTS OF HUMAN TEMPOROMANDIBULAR JOINTS
PRESENCE OF TYPE-III COLLAGEN IN DISK ATTACHMENTS OF HUMAN TEMPOROMANDIBULAR JOINTS
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DOI：
10.1016/0003-9969(90)90044-b
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发表时间：
1990-01-01
影响因子：
3
通讯作者：
FRANCIS, MJO
中科院分区：
文献类型：
--
作者：
GAGE, JP;VIRDI, AS;FRANCIS, MJO
通过解离方法提取椎间盘和椎间盘附件的样品，并用溴化氰裂解所得的胶原残留物。由此释放的可溶性肽的特征在于它们的电泳迁移率后SDS-PAGE和免疫印迹染色的特异性抗体对I型和III型胶原蛋白。在取自后椎间盘附件的样本中鉴定出III型胶原。这可能解释了为什么这个盘容易脱离和内部紊乱以及颞下颌关节功能障碍患者的高发病率。
Samples of discs and disc attachments were extracted by dissociative methods and the resultant collagenous residues cleaved with cyanogen bromide. Soluble peptides thus released were characterized by their electrophoretic mobility following SDS-PAGE and by Western blot staining with specific antibodies against type I and III collagens. Type III collagen was identified in samples taken from the posterior discal attachments. This may explain why this disc is prone to detachment and internal derangement and the high incidence of patients with temporomandibular joint dysfunction.