The YABBY Gene DROOPING LEAF Regulates Carpel Specification and Midrib Development in Oryza sativa On-line version contains Web-only data.
The YABBY Gene DROOPING LEAF Regulates Carpel Specification and Midrib Development in Oryza sativa On-line version contains Web-only data.
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DOI：
10.1105/tpc.018044
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发表时间：
2004-02
期刊：
影响因子：
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通讯作者：
Takahiro Yamaguchi;N. Nagasawa;S. Kawasaki;M. Matsuoka;Y. Nagato;H. Hirano
中科院分区：
文献类型：
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作者：
Takahiro Yamaguchi;N. Nagasawa;S. Kawasaki;M. Matsuoka;Y. Nagato;H. Hirano
在这篇文章中，我们报道了水稻花的心皮特性是由花同源异型基因下垂叶(DL)调控的，该基因不同于众所周知的ABC基因。严重的DL功能丧失突变导致心皮到雄蕊的完全同源同源转化。分子克隆表明，该基因是Yabby基因家族的成员，与拟南芥的蟹爪基因(CRC)关系密切。Dl在心皮原基起始的推测区域(心皮原基)和心皮原基中表达。这些结果表明，水稻花发育过程中心皮规格受DL调控。虽然CRC在心皮鉴定中只起到部分作用，但在水稻进化过程中，DL可能已经被招募来具有更基本的功能，即指定心皮。我们还表明，DL与B类基因有拮抗作用，并控制花分生组织的决定性。此外，强dl等位基因和弱dl等位基因不能在叶片中形成中脉。对dl突变体的表型分析，以及对dl的空间表达模式和异位表达的分析表明，dl通过促进水稻叶片中央区域的细胞增殖来调节中脉的形成。
In this article, we report that carpel specification in the Oryza sativa (rice) flower is regulated by the floral homeotic gene DROOPING LEAF (DL) that is distinct from the well-known ABC genes. Severe loss-of-function mutations of DL cause complete homeotic transformation of carpels into stamens. Molecular cloning reveals that DL is a member of the YABBY gene family and is closely related to the CRABS CLAW (CRC) gene of Arabidopsis thaliana. DL is expressed in the presumptive region (carpel anlagen), where carpel primordia would initiate, and in carpel primordia. These results suggest that carpel specification is regulated by DL in rice flower development. Whereas CRC plays only a partial role in carpel identity, DL may have been recruited to have the more essential function of specifying carpels during the evolution of rice. We also show that DL interacts antagonistically with class B genes and controls floral meristem determinacy. In addition, severe and weak dl alleles fail to form a midrib in the leaf. The phenotypic analysis of dl mutants, together with analyses of the spatial expression patterns and ectopic expression of DL, demonstrate that DL regulates midrib formation by promoting cell proliferation in the central region of the rice leaf.