Extended storage of red blood cells under anaerobic conditions
Extended storage of red blood cells under anaerobic conditions
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DOI：
10.1111/j.1423-0410.2006.00860.x
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发表时间：
2007-01-01
期刊：
影响因子：
2.7
通讯作者：
Bitensky, M. W.
中科院分区：
文献类型：
--
作者：
Yoshida, T.;AuBuchon, J. P.;Bitensky, M. W.
背景红细胞（RBC）在冷藏过程中会受到活性氧的氧化应激。在储存过程中几乎完全去除红细胞中的氧，应该可以消除红细胞“储存损伤”的贡献者。在体外储存红细胞缺氧条件下延长时间的影响进行了研究，这些与所观察到的回收率reinfus.Study设计和方法单位的红细胞，获得后“软旋转”，被放置在一个双体积的AS-3添加剂的解决方案和细分。通过反复暴露于Ar气（至O-2饱和度< 4%）耗尽测试单元中的氧气，并将单元储存在厌氧罐中长达15周。每周采集样本以监测三磷酸腺苷（ATP）、2，3-二磷酸甘油酸（2，3-DPG）、无细胞血红蛋白和囊泡产生。在一项平行实验中，以类似的方式耗尽6个单位的红细胞的氧气，储存8周、9周和10周，并自体回输，以通过Cr-51/Tc-99 m放射性标记确定输血后24小时的回收率。一个类似的研究也进行了使用EAS 61添加剂的解决方案，这本身，已经显示出支持9周的存储能力，比较生化档案和体内恢复后有氧与厌氧storage.Results缺氧AS-3单位有显着升高的ATP水平相比，控制。当红细胞储存超过9周时，它们的无细胞血红蛋白和囊泡产量也显著降低。在厌氧储存9周后观察到平均超过75%的输血后存活率，溶血率低于0.43%。然而，没有进一步延长存储实现与EAS 61 additive.Conclusion厌氧条件下允许使用双倍体积AS-3添加剂溶液的红细胞可接受的9周的存储。它没有与碱性的9周添加剂EAS 61协同作用，以进一步延长可接受的储存时间。这些研究表明，厌氧储存可能会减少储存损伤的影响，但也表明其他因素也会导致RBC储存的限制。
Background Red blood cells (RBC) are subject to oxidative stress by reactive oxygen species during refrigerated storage. Near-complete removal of oxygen from red cells during storage should eliminate this contributor to the red cell 'storage lesion'. The in vitro effects of storing red cells under oxygen-depleted conditions for extended periods were investigated, and these were correlated with the observed recoveries after reinfusion.Study Design and Methods Units of red cells, obtained after 'soft spin', were placed in a double volume of AS-3 additive solution and subdivided. Oxygen in the test units was depleted by repeated exposure to Ar gas (to O-2 saturation < 4%), and units were stored in anaerobic canisters for up to 15 weeks. Samples were taken weekly to monitor adenosine triphosphate (ATP), 2,3-diphosphoglycerate (2,3-DPG), cell-free haemoglobin, and vesicle production. In a parallel experiment, six units of red cells was depleted of oxygen in a similar manner, stored for 8, 9 and 10 weeks, and reinfused autologously to determine the 24 h post-transfusion recovery via Cr-51/Tc-99m radiolabelling. A similar study was also carried out using EAS61 additive solution, which by itself, had shown the ability to support 9-week storage, comparing biochemical profiles and in vivo recovery after aerobic vs. anaerobic storage.Results Oxygen-depleted AS-3 units had significantly elevated ATP levels compared to controls. They also had significantly lower cell free haemoglobin and vesicle production when RBCs were stored for more than 9 weeks. An average of over 75% post-transfusion survival was observed after 9 weeks of anaerobic storage with less than 0.43% haemolysis. However, no further extension of storage was achieved with EAS61 additive.Conclusion Anaerobic conditions permit acceptable 9-week storage of RBCs using double-volume AS-3 additive solution. It did not synergize with the alkaline, 9-week additive, EAS61, to further lengthen the acceptable storage time. These studies indicate that anaerobic storage may allow reduction in the effect of the storage lesion, but suggest that other factors contribute to limitations of RBC storage as well.