Local activation of TGF-beta1 at endometriosis sites.
Local activation of TGF-beta1 at endometriosis sites.
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发表时间：
2007
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通讯作者：
S. Komiyama;D. Aoki;M. Komiyama;S. Nozawa
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作者：
S. Komiyama;D. Aoki;M. Komiyama;S. Nozawa
目的探讨子宫内膜异位症患者子宫内膜组织中转化生长因子-β 1（TGF-β 1）活化的相关因素。研究设计TGF-β 1被纤溶酶激活，纤溶酶是纤溶酶原被尿激酶型纤溶酶原激活剂（uPA）激活后形成的。我们通过免疫组织化学或免疫测定来研究这些因素。结果TGF-β 1蛋白在子宫内膜异位囊肿和正常子宫内膜中主要定位于腺上皮细胞胞浆，但在囊肿中强阳性表达明显增多。囊肿液中TGF-β 1、uPA和纤溶酶/α 2-纤溶酶抑制剂复合物的水平均高于腹腔液。uPA蛋白在正常子宫内膜腺上皮中表达较少，但在增生性子宫内膜异位囊肿腺上皮细胞胞浆中表达较强，且在囊肿中强阳性表达明显增多。结论子宫内膜异位症组织中TGF-β 1活性增高，可能是由于腺上皮分泌uPA和TGF-β 1的增加以及纤溶酶原被uPA转化为纤溶酶后激活TGF-β 1。
OBJECTIVE To investigate the factors related to activation of transforming growth factor-beta 1 (TGF-beta1) at sites of endometriosis. STUDY DESIGN TGF-beta1 is activated by plasmin, which is formed when plasminogen is activated by urokinase-type plasminogen activator (uPA). We studied these factors by immunohistochemistry or immunoassay. RESULTS TGF-beta1 protein was localized mainly in the cytoplasm of glandular epithelial cells in both endometriotic cysts and normal endometrium, but strongly positive immunostaining was significantly more common in cysts. The levels of TGF-beta1, uPA and plasmin/alpha2-plasmin inhibitor complex were all higher in cyst fluid than in peritoneal fluid. There was little uPA protein expression in the glandular epithelium of normal endometrium, but it was prominent in the cytoplasm of glandular epithelial cells from endometriotic cysts, and strongly positive immunostaining was significantly more common in cysts. CONCLUSION These results suggest that TGF-beta1 activity is increased at sites of endometriosis due to enhanced production of both uPA and TGF-beta1 by glandular epithelium and because plasmin activates TGF-beta1 after being converted from plasminogen by uPA.