Over-Expression of CDH22 Is Associated with Tumor Progression in Colorectal Cancer
Over-Expression of CDH22 Is Associated with Tumor Progression in Colorectal Cancer
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DOI：
10.1159/000225242
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发表时间：
2009-06
期刊：
影响因子：
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通讯作者：
Jun Zhou;Jian-ming Li;Juan-zhi Chen;Yuhong Liu;Wei-zhe Gao;Yanqing Ding
中科院分区：
文献类型：
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作者：
Jun Zhou;Jian-ming Li;Juan-zhi Chen;Yuhong Liu;Wei-zhe Gao;Yanqing Ding
CDH22是钙粘蛋白家族的一员，在脑垂体和大脑中高度表达。此前已发现CDH22参与脑和神经内分泌器官的神经细胞和非神经细胞的形态发生和组织形成。然而，人们对其在结直肠癌中的作用知之甚少。本实验检测了CDH22在结直肠肿瘤中的作用，并探讨了抑制CDH22表达对结直肠肿瘤增殖和转移的影响。我们发现，与正常结肠粘膜相比，CDH22在结直肠癌和结直肠癌淋巴转移中转录和翻译水平均过表达。CDH22在蛋白和mRNA水平上的过表达与结直肠癌的侵袭转移均有显著相关性。在体内和体外实验中，敲除CDH22可部分阻断结直肠癌细胞的增殖。在结肠手术原位植入小鼠模型中，敲低CDH22足以减弱结直肠癌细胞的侵袭并抑制肿瘤转移。我们的研究结果首次揭示了CDH22在结直肠癌进展中的新作用。
CDH22, a member of the cadherins family, is highly expressed in the pituitary gland and the brain. It has been previously found that CDH22 is involved in morphogenesis and tissue formation in neural and non-neural cells of the brain and neuroendocrine organ. However, little is known about its role in colorectal cancer. Here the role of CDH22 in colorectal tumor was tested, and effects of inhibition of CDH22 expression on proliferation and metastasis were examined. We found that CDH22 was over-expressed at both transcriptional and translational levels in colorectal cancer and lymphatic metastasis of colorectal carcinoma, compared with normal colorectal mucosa. CDH22 over-expression was significantly associated with invasion and metastasis of colorectal cancer both at protein and mRNA levels. CDH22 knockdown partially blocked proliferation of colorectal cancer cells in vivo and in vitro. CDH22 knockdown was sufficient to attenuate invasion of colorectal cancer cells and inhibit tumor metastasis in a mouse model of colon surgical orthotopic implantation. Our results reveal for the first time a new role of CDH22 in progression of colorectal cancer.