Stem cell homing factor, CCL7, expression in mouse models of stress urinary incontinence.
Stem cell homing factor, CCL7, expression in mouse models of stress urinary incontinence.
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DOI：
10.1097/spv.0b013e3182a331a9
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发表时间：
2013-11
影响因子：
1.6
通讯作者：
Daneshgari F
中科院分区：
文献类型：
--
作者：
Hijaz AK;Grimberg KO;Tao M;Schmotzer B;Sadeghi Z;Lin YH;Kavran M;Ozer A;Xiao N;Daneshgari F
阴道扩张（VD）动物模型已表明趋化因子（C - C基序）配体7（CCL7）表达增加。在本研究中，我们利用阴道扩张和阴部神经横断（PNT），研究了模拟分娩创伤诱导的尿失禁小鼠模型中CCL7的表达。
49只小鼠被分为6组：阴道扩张组、假阴道扩张组、阴部神经横断组、假阴部神经横断麻醉组以及年龄匹配的对照组。在指定操作后立即或24小时采集尿道、阴道和直肠以检测CCL7的表达。还进行了静脉采血以定量检测血清CCL7。采用方差分析模型比较每组中CCL7的相对表达。
24小时后，阴道扩张组的尿道CCL7表达显著高于对照组（p < 0.01）。与对照组相比，阴部神经横断组、假阴部神经横断组、假阴道扩张组或麻醉组的尿道CCL7表达没有差异。除假阴部神经横断组外，任何组之间阴道和直肠的CCL7表达均未发现有统计学意义的差异。24小时后，与对照组相比，阴道扩张组、阴部神经横断组和假阴部神经横断组的血清CCL7表达有统计学意义的差异（均p < 0.01）。
本研究表明阴道扩张后尿道CCL7过度表达，但阴部神经横断后没有。这表明神经损伤对CCL7过度表达没有影响。阴道扩张后小鼠血清中CCL7的过度表达提示了一种转化潜力，即CCL7的测量可作为分娩后损伤的替代指标。
Animal models of vaginal distention (VD) have demonstrated increased expression of chemokine (C-C motif) ligand 7 (CCL7). In this study, we investigated the expression of CCL7 in mice models of simulated birth trauma-induced urinary incontinence utilizing VD and pudendal nerve transection (PNT). Forty-nine mice were divided into 6 groups: VD, sham VD, PNT, sham PNT anesthesia, and age-matched controls. The urethra, vagina, and rectum were harvested for the expression of CCL7 immediately or 24 hours after assigned procedure. Venous sampling for quantification of serum CCL7 was also performed. An ANOVA model was used to compare the relative expression of CCL7 in each group. Urethral CCL7 expression in the VD group was significantly higher than control group after 24 hours (p<0.01). There was no difference in the urethral CCL7 expression in PNT, sham PNT, sham VD or anesthesia groups compared with controls. No statistically significant difference was noted in the vaginal and rectal expression of CCL7 between any of the groups except for sham PNT. Statistically significant differences were noted in the serum CCL7 expression in VD, PNT and sham PNT (p<0.01 in all) groups after 24 hours compared with the control group. This study demonstrates over-expression of urethral CCL7 after VD but not PNT. This suggests that nerve injury does not contribute to the CCL7 over-expression. The over-expression of CCL7 in the serum of mice after VD suggests a translational potential where CCL7 measurement could be used as a surrogate for injury after delivery.