Abnormal clones of T cells producing interleukin-5 in idiopathic eosinophilia
Abnormal clones of T cells producing interleukin-5 in idiopathic eosinophilia
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DOI：
10.1056/nejm199910073411503
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发表时间：
1999-10-07
影响因子：
158.5
通讯作者：
Blaser, K
中科院分区：
文献类型：
--
作者：
Simon, HU;Plötz, SG;Blaser, K
背景：持续性嗜酸性粒细胞增多症和嗜酸性粒细胞增多综合征的病因尚不清楚。最近的研究表明，在一些嗜酸性粒细胞增多综合征患者中，异常T细胞的克隆产生大量的白细胞介素-5，这是嗜酸性粒细胞生长和分化所需的细胞因子。我们研究了T细胞表面标志物，重排的T细胞受体基因，并在体外生产的细胞因子T细胞从特发性eosinophility.Methods患者的T细胞表面分子的表达进行了测定，通过流式细胞术。通过酶联免疫吸附试验、流式细胞术和免疫组织化学分析测定细胞因子表达。为了确定显性（克隆）重排的T细胞受体内的淋巴细胞群体，Southern印迹分析（β链）和聚合酶链反应（γ链）进行根据标准protocols.Results 60例特发性嗜酸性粒细胞增多症，16例循环T细胞与异常的免疫表型。在这些患者中，异常免疫表型是独特的。在16例患者中的8例中获得了T细胞受体克隆重排的证据。在大多数情况下，异常T细胞表达大量与T细胞活化相关的表面蛋白（白细胞介素-2受体的α链和HLA-DR抗原）。结论特发性嗜酸性粒细胞增多症患者存在产生IL-5的异常T细胞克隆群。（N Engl J Med 1999;341：1112-20.）（C）1999年，马萨诸塞州医学会。
Background The cause of persistent eosinophilia and the hypereosinophilic syndrome is unknown. Recent work suggests that in some patients with the hypereosinophilic syndrome, a clone of abnormal T cells produces large amounts of interleukin-5, a cytokine required for the growth and differentiation of eosinophils. We examined T-cell surface markers, rearranged T-cell-receptor genes, and in vitro production of cytokines by T cells from patients with idiopathic eosinophilia.Methods The expression of surface molecules on T cells was measured by flow cytometry. Cytokine expression was measured by enzyme-linked immunosorbent assay, flow cytometry, and immunohistochemical analysis. To identify dominant (clonal) rearrangements of the T-cell receptor within the lymphocyte population, Southern blot analysis (beta chain) and the polymerase chain reaction (gamma chain) were performed according to standard protocols.Results Among 60 patients with idiopathic eosinophilia, 16 had circulating T cells with an aberrant immunophenotype. In each of these patients, the abnormal immunophenotype was unique. Evidence of clonal rearrangements of the T-cell receptor was obtained in 8 of the 16 patients. In most instances, the abnormal T cells expressed large amounts of surface proteins associated with T-cell activation (the a chain of the interleukin-2 receptor and the HLA-DR antigen). Moreover, the aberrant T cells produced large amounts of interleukin-5 in vitro.Conclusions Clonal populations of abnormal T cells producing interleukin-5 occur in some patients with idiopathic eosinophilia. (N Engl J Med 1999;341:1112-20.) (C)1999, Massachusetts Medical Society.