Metabolomic and proteomic changes in the xylem sap of maize under drought
Metabolomic and proteomic changes in the xylem sap of maize under drought
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DOI：
10.1111/j.1365-3040.2007.01770.x
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发表时间：
2008-03-01
影响因子：
7.3
通讯作者：
Schachtman, Daniel P.
中科院分区：
文献类型：
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作者：
Alvarez, Sophie;Marsh, Ellen L.;Schachtman, Daniel P.
植物在根中产生化合物，通过木质部汁液输送到芽中，其中一些化合物对信号传导和适应环境胁迫（如干旱）至关重要。在这项研究中，我们使用质谱法筛选木质部汁液，以量化在长期干旱下新的和以前鉴定的汁液成分的变化。我们检测并量化了在逐渐增加的干旱胁迫下木质部汁液中存在的31种化合物浓度的变化。我们发现了脱落酸（ABA）和细胞分裂素的变化，以及高浓度的芳香细胞分裂素6-苄基氨基嘌呤（BAP）的存在。在木质部液中发现了几种苯丙酸类化合物（香豆酸、咖啡酸和阿魏酸），其中一些苯丙酸类化合物的浓度在干旱条件下发生了变化。同时，对木质部汁液蛋白质组进行了分析。我们发现阳离子过氧化物酶的丰度较高，随着苯丙素含量的增加，阳离子过氧化物酶可能导致木质部血管中木质素生物合成的减少，并可能诱导细胞壁硬化。新方法的应用提供了对树液中化合物范围的见解，以及成分的变化如何导致适应干旱过程中发育和信号的变化。
Plants produce compounds in roots that are transported to shoots via the xylem sap. Some of these compounds are vital for signalling and adaptation to environmental stress such as drought. In this study, we screened the xylem sap using mass spectrometry to quantify the changes in new and previously identified sap constituents under extended drought. We detected and quantified the changes in the concentration of 31 compounds present in the xylem sap under progressively increasing drought stress. We found changes in the hormones abscisic acid (ABA) and cytokinin, and the presence of high concentrations of the aromatic cytokinin 6-benzylaminopurine (BAP). Several phenylpropanoid compounds (coumaric, caffeic and ferulic acids) were found in xylem sap. The concentrations of some of these phenylpropanoid compounds changed under drought. In parallel, an analysis of the xylem sap proteome was conducted. We found a higher abundance of cationic peroxidases, which with the increase in phenylpropanoids may lead to a reduction in lignin biosynthesis in the xylem vessels and could induce cell wall stiffening. The application of new methodologies provides insights into the range of compounds in sap and how alterations in composition may lead to changes in development and signalling during adaptation to drought.