Comparative RNA-sequencing analysis of mthl1 functions and signal transductions in Tribolium castaneum
Comparative RNA-sequencing analysis of mthl1 functions and signal transductions in Tribolium castaneum
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赤拟谷盗 mthl1 功能和信号转导的比较 RNA 测序分析
DOI：
10.1016/j.gene.2014.06.064
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发表时间：
2014-09-01
期刊：
影响因子：
3.5
通讯作者：
Li, Bin
中科院分区：
文献类型：
--
作者：
Li, Chengjun;Wu, Wei;Li, Bin
据报道，卡氏拟方法学1(TcMthl1)在植物的发育、寿命、抗逆性和生殖等方面发挥着重要作用。然而，TcMthl1的信号系统尚不清楚。因此，我们通过RNA测序比较了RNAi处理的卡氏锥虫幼虫(DS-Tcmthl1)和对照幼虫的转录组图谱，获得了14,613,514个序列读数，与13,533个基因序列一致，鉴定出812个差异表达基因(Deg)。这些DEG被分为47个GO功能群，包括免疫系统过程、对刺激的反应、发育过程和生殖等功能。有趣的是，Tcmthl1的敲除抑制了Toll和IMD免疫通路，这很可能调节了Tcmthl1对寿命和抗应激能力的影响。此外，编码Blimp-1、FTZ-F1、E74和Timless的DEG可能参与了DS-Tcmthl1昆虫的发育和繁殖。这些基因和通路的发现将为深入研究TcMthl1信号和调控系统提供有价值的信息。(C)2014爱思唯尔B.V.保留所有权利。
Methuselah-like 1 of Tribolium castaneum (TcMthl1) has been reported to play crucial roles in development, lifespan, stress resistance and reproduction. However, the signaling system of TcMthl1 is unknown. Thus, we compare the transcriptome profile of RNAi treated larvae (ds-Tcmthl1) and control larvae of T. castaneum by RNA-sequencing, and obtained 14,613,514 sequence reads aligned with 13,533 genes; 812 differentially expressed genes (DEGs) were identified. These DEGs were classified into 47 GO functional groups, including such functions as the immune system process, the response to stimulus, the developmental process and reproduction. Interestingly, knock-down of Tcmthl1 suppressed both of Toll and IMD immunity pathways which most likely modulated the effects of Tcmthl1 on lifespan and stress resistance. Additionally, the DEGs encoding Blimp-1, Ftz-F1, E74 and Timeless may participate in the development and reproduction of ds-Tcmthl1 insects. The findings of these DEGs and pathways will provide valuable insight into TcMthl1 signaling and regulation system. (C) 2014 Elsevier B.V. All rights reserved.