Microbacterium populi sp. nov., isolated from Populus×euramericana bark.
Microbacterium populi sp. nov., isolated from Populus×euramericana bark.
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DOI：
10.1099/ijs.0.000117
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发表时间：
2015-05
影响因子：
2.8
通讯作者：
Yong Li;Tao Wang;W. Fang;H. Xue;C. Piao;M. Guo;T. Zhu
中科院分区：
文献类型：
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作者：
Yong Li;Tao Wang;W. Fang;H. Xue;C. Piao;M. Guo;T. Zhu
从河南省濮阳市的欧美杨树皮中分离到5株革兰氏染色阳性的好氧菌，分别为10-107-8（T）、1C-4、NHI 3_6、4107_1_2和3D-3。分离物在15-40 °C和pH 5-10下生长。最适生长温度为30 °C，最适生长pH为8.0。化学分类学特征包括MK-10和MK-11作为主要的甲基萘醌（模式菌株）;主要的异支化和反异支化细胞脂肪酸;二磷脂酰甘油和磷脂酰甘油作为主要的极性脂质（模式菌株）;鸟氨酸作为细胞壁肽聚糖的主要二氨基酸（模式菌株）;乙醇酰基型作为细胞壁酰基型; DNA G+C含量为66.8-67.6 mol%。这些特征与微杆菌属的分类一致。16 S rRNA基因序列分析表明，5株菌均属于微杆菌属，与耐盐微杆菌（Microbacterium halotolerans）亲缘关系较近。16 S rRNA基因与M.耐盐菌YIM 70130（T）。这5株菌与微杆菌属其他已发表的菌种的16 SrRNA基因序列相似性均小于96.20%。5个分离物与M.耐盐菌JCM 13013（T）的耐盐率为35.62%~ 44.36%。综合16 S rRNA基因序列分析结果和生理生化特征，我们认为这5株菌属于微杆菌属的一个新种。这五种菌株被命名为杨微杆菌，模式菌株为10-107-8（T）（=CFCC 11275（T）=KCTC 29152（T））。
Five non-spore-forming, aerobic and Gram-stain-positive bacterial strains, 10-107-8(T), 1C-4, NHI3_6, 4107_1_2, and 3D-3, were isolated from Populus × euramericana bark collected in Puyang City, Henan Province, PR China. The isolates grew at 15-40 °C and pH 5-10. The optimum temperature and pH for growth were 30 °C and pH 8.0, respectively. Chemotaxonomic features included MK-10 and MK-11 as major menaquinones (type strain); predominating iso- and anteiso-branched cellular fatty acids; diphosphatidylglycerol and phosphatidylglycerol as major polar lipids (type strain); ornithine as the principal diamino acid of the cell-wall peptidoglycan (type strain); glycolyl type as cell-wall acyl type; and DNA G+C content of 66.8-67.6 mol%. These features were consistent with classification in the genus Microbacterium . Analysis of 16S rRNA gene sequence data indicated that the five isolates belonged to the genus Microbacterium and were closely related to Microbacterium halotolerans . A high 16S rRNA gene sequence similarity of 96.97% to M. halotolerans YIM 70130(T) was observed. The five isolates showed less than 96.20% 16S rRNA gene sequence similarity to the other species of the genus Microbacterium with validly published names. DNA-DNA relatedness of the five isolates with M. halotolerans JCM 13013(T) ranged from 35.62% to 44.36%. Considering the results of 16S rRNA gene sequence analysis and the physiological and biochemical characteristics, we propose that the five strains should be assigned to a novel species of the genus Microbacterium . The name proposed for the five strains is Microbacterium populi sp. nov., and the type strain is 10-107-8(T) ( =CFCC 11275(T) =KCTC 29152(T)).